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Biopsy specimen identification by detection of sex chromosomes: application of in situ hybridisation.
  1. R E Kibbelaar,
  2. C F Leenheers-Binnendijk,
  3. P J Spaander,
  4. P M Kluin
  1. Laboratory of Pathology, State University Leiden, The Netherlands.

    Abstract

    AIMS: To investigate the feasibility of non-radioactive in situ hybridisation (ISH) for the identification of sex-mismatched plastic embedded bone marrow biopsy specimens. METHODS: After a suspected accidental transposition of two glycol-methacrylate embedded bone marrow specimens, in situ hybridisation with sex chromosome specific probes was performed. RESULTS: Quantitative analysis of the hybridisation signals established unequivocably the origin of the specimens. CONCLUSIONS: ISH is feasible on GMA embedded bone marrow specimens, and can be used for the identification of accidentally transposed specimens provided that they are of sex-matched origin.

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