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Detection of immunoglobulin heavy chain gene rearrangements in Hodgkin's disease using PCR.
  1. C A Angel,
  2. J H Pringle,
  3. L Primrose,
  4. I Lauder
  1. Department of Pathology, University of Sheffield Medical School.

    Abstract

    AIM--To detect clonal rearrangements of the immunoglobulin (Ig) heavy chain gene in Hodgkin's disease tissue using the polymerase chain reaction (PCR). METHODS--DNA extracted from 36 samples of Hodgkin's disease was analysed using PCR and primers from conserved sequences in the variable (VH) and joining (JH) regions. RESULTS--Clonal rearrangement was detected only in one case. Evidence of clonal immunoglobulin gene rearrangement had been detected previously in this case using conventional Southern blot analysis. CONCLUSIONS--The sensitivity of the two techniques is equivalent and clonal Ig heavy chain gene rearrangements are rare in Hodgkin's disease.

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