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Latex agglutination for rapid detection of Pseudomonas pseudomallei antigen in urine of patients with melioidosis.
  1. M D Smith,
  2. V Wuthiekanun,
  3. A L Walsh,
  4. N Teerawattanasook,
  5. V Desakorn,
  6. Y Suputtamongkol,
  7. T L Pitt,
  8. N J White
  1. Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

    Abstract

    A latex agglutination test for the detection of Pseudomonas pseudomallei antigen in urine was evaluated for the rapid diagnosis of melioidosis. With unconcentrated urine, antigen was detected in only 18% of patients with melioidosis overall. However, when urine was concentrated 100-fold, antigen was detected in 47% overall and in 67% of patients with septicaemia or disseminated infection, in whom a rapid diagnosis is most important. The specificity of the test was 100%. These results compared favourably with an enzyme immunoassay. This latex agglutination test is a simple, rapid and highly specific method of diagnosing melioidosis, and will be particularly useful in areas with limited laboratory facilities.

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