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Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis.
  1. P J Horner,
  2. M Ali,
  3. D Parker,
  4. J N Weber,
  5. D Taylor-Robinson,
  6. M O McClure
  1. Department of Genito-urinary Medicine and Communicable Diseases, Imperial College School of Medicine at St Mary's, London.

    Abstract

    AIMS: To develop an indirect ELISA using the heat shock protein (hsp60) of Chlamydia trachomatis as antigen. METHODS: The hsp60 gene was amplified by PCR, expressed in the vector pDEV-107 and transformed into Escherichia coli. The recombinant protein, expressed as a beta-galactosidase fusion product, was captured onto a solid phase using a monoclonal antibody directed against beta-galactosidase. Following incubation with goat anti-human antibody conjugated to peroxidase and colour development on addition of peroxidase substrate, antibody recognition of antigen was quantified by optical density at 492 nm. RESULTS: A sensitive and relatively specific ELISA to detect hsp60 has been produced, which can be exploited to determine the antibody response to C trachomatis hsp60. CONCLUSIONS: This assay will permit the future investigation of the immunopathogenesis of persistent inflammation following C trachomatis infection.

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