You are here

  • Home
  • Archive
  • Volume 54, Issue 4
  • MPO-ANCA may produce a combination of P-ANCA and atypical cytoplasmic ANCA indirect immunofluorescent patterns on certain ethanol fixed neutrophil substrates

Article Text

Article menu
Download PDFPDF
MPO-ANCA may produce a combination of P-ANCA and atypical cytoplasmic ANCA indirect immunofluorescent patterns on certain ethanol fixed neutrophil substrates
  1. R C W Wong,
  2. K Field
  1. Division of Immunology, Queensland Health Pathology Services, Level 3, Lions Pathology Building, Princess Alexandra Hospital, Woolloongabba 4102, Queensland, Australia richard_wong@health.qld.gov.au

    https://doi.org/10.1136/jcp.54.4.335

    Statistics from Altmetric.com

      Request Permissions

      If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

      The P-ANCA pattern is defined as perinuclear indirect immunofluorescent (IIF) staining on ethanol fixed normal human neutrophils.1–3 This pattern is an artefact of ethanol fixation, dependent on the redistribution of certain cationic neutrophil granule proteins (such as myeloperoxidase (MPO), lactoferrin, and lysozyme) around the negatively charged nuclear membrane.1, 2 However, certain MPO-ANCA can produce cytoplasmic rather than perinuclear IIF staining,4 possibly related to a subpopulation of epitopes on MPO that do not redistribute with ethanol fixation.2 We now report that MPO-ANCA positive sera may produce a combination of P-ANCA and atypical cytoplasmic5 ANCA IIF patterns on certain ethanol fixed neutrophil substrates, potentially leading to interpretative and diagnostic difficulties.

      Sera from six patients …

      View Full Text

      Linked Articles