Article Text

PDF

Diagnostic role of tests for T cell receptor (TCR) genes
  1. E Hodges,
  2. M T Krishna,
  3. C Pickard,
  4. J L Smith
  1. Wessex Immunology, Southampton University Hospitals NHS Trust, Tremona Road, Southampton SO16 6YD, UK
  1. Correspondence to:
 Dr E Hodges, Wessex Immunology, Southampton University Hospital NHS Trust, Tremona Road, Southampton SO16 6YD, UK;
 elizabethhodges{at}hotmail.com

Abstract

Rapid advances in molecular biological techniques have made it possible to study disease pathogenesis at a genomic level. T cell receptor (TCR) gene rearrangement is an important event in T cell ontogeny that enables T cells to recognise antigens specifically, and any dysregulation in this complex yet highly regulated process may result in disease. Using techniques such as Southern blot hybridisation, polymerase chain reaction, and flow cytometry it has been possible to characterise T cell proliferations in malignancy and in diseases where T cells have been implicated in the pathogenesis. The main aim of this article is to discuss briefly the process of TCR gene rearrangement and highlight the disorders in which expansions or clonal proliferations of T cells have been recognised. It will also describe various methods that are currently used to study T cell populations in body fluids and tissue, their diagnostic role, and current limitations of the methodology.

  • T cells
  • T cell receptor gene rearrangement
  • clonality
  • polymerase chain reaction
  • flow cytometry
  • T cell receptor repertoire
  • B-ALL, B cell acute lymphoblastic leukaemia
  • C, constant
  • CDR, complementarity determining regions
  • CMV, cytomegalovirus
  • CVID, common variable immunodeficiency
  • D, diversity
  • DGGE, denaturing gradient gel electrophoresis
  • EBV, Epstein-Barr virus
  • HIV, human immunodeficiency virus
  • HLA, human major histocompatibility complex
  • HTLV, human T cell lymphotropic virus
  • Ig, immunoglobulin
  • LGL, large granular lymphocytosis
  • J, joining
  • MHC, major histocompatibility complex
  • MRD, minimal residual disease
  • PCR, polymerase chain reaction
  • PTCL, peripheral T cell lymphoma
  • RSS, recombination signal sequences
  • RT, reverse transcription
  • SCID, severe combined immunodeficiency
  • SSCP, single strand conformation polymorphism
  • TCR, T cell receptor
  • TdT, terminal deoxynucleotidyl transferase
  • V, variable

Statistics from Altmetric.com

The T cell receptor (TCR) is a membrane bound heterodimer composed of two polypeptide chains (αβ or γδ) linked by a disulfide bond and associated with a non-polymorphic cytoplasmic membrane bound complex of proteins collectively known as CD3.1–3 In the peripheral blood, most T cells express the αβ receptor and up to 15% express the γδ receptor.4–6 The extracellular domains of the TCR consist of a constant (C) domain and a variable (V) domain. Hypervariable regions or complementarity determining regions (CDR) encoded within the TCR V domains contact the peptide antigen and the major histocompatibility (MHC) molecules and therefore confer the specificity of the TCR. Furthermore, T cells are MHC restricted in that CD4 positive or helper T cells recognise peptides bound to MHC class II molecules and CD8 positive or suppressor T cells recognise peptides bound to MHC class I molecules.

THE TCR LOCI

The TCR genes are located on chromosomes 7 and 14. The genes encoding the TCRα (TCRD) chain are located within the TCRα (TCRA) loci on chromosome 14q11–12, whereas the TCRβ (TCRB) and TCRγ (TCRG) genes are located at chromosomal positions 7q32–35 and 7p15, respectively (fig 1; table 1).8–14 The four genes are composed of C and V regions which are assembled together during thymic ontogeny by somatic recombination.15 Briefly, the V domains in the TCRA and TCRG chains are assembled from V and J gene segments whereas the TCRB and TCRD chains are assembled from V, diversity (D), and J segments. The multiple non-contiguous V, D, and J segments undergo rearrangement during T cell development to form complete V domain exons, which are responsible for antigen recognition. Diversity of the T cell repertoire is not only a consequence of different V, D, J recombinations but also results from nucleotide insertion and deletion at junctional sites (V–J, D–J, and V–D), thereby increasing diversity for recognition of a vast array of antigens.

Table 1

Genes encoding the T cell receptor (TCR) chains

Figure 1

Germline organisation of the T cell receptor (TCR) genes. The TCRA, TCRB, and TCRG loci are located on the chromosomes at positions 14q11–12, 7q32–35, and 7p15, respectively. The TCRD loci are also situated on chromosome 14, within the TCRA loci. The gene segments are designated using the World Health Organisation standards for TCR nomenclature.7 The shaded boxes indicate the type of gene segment: V, variable (black); D, diversity (white); J, joining (dark grey); C, constant (light grey).

“The multiple non-contiguous V, D, and J segments undergo rearrangement during T cell development to form complete V domain exons, which are responsible for antigen recognition”

The TCRA genes consist of 70 V gene segments, divided into 41 families16–19 and at least 61 J segments spread over 800 kb upstream from a single C gene. The 61 J gene segments, consisting of 50 functional J genes, are not clustered and are located at over 65 kb at various distances 5′ of the C gene.14,20

The TCRB locus comprises approximately 67 V genes of which 47 are functional and 19 are pseudogenes.21 The functional TCRBV families are grouped into 23 families based upon greater than 75% nucleotide sequence identity.22 Two C genes are associated with upstream clusters of J genes and a single D gene (D1 or D2) and are known as the D–J–C clusters. There are 13 J genes, designated J1S1 to J1S6 and J2S1 to J2S7.23

TCRG consists of up to 14 V segments and a duplicated J–C cluster consisting of three J genes (J1S1, J1S2, and J1S3) and one C gene, and two J genes (J2S1 and J2S3) and one C gene, respectively. The 14 V genes belong to four families. The TCRGVI family consists of nine members, five of which are functional and four of which are pseuodogenes, and the remaining three families (II, III, and IV) each consist of a single gene. The remaining two genes are VA, which lies 5′ to the TCRGVII, and VB, which lies 5′ to TCRGVIII.24,25

The TCRD locus is located within the TCRA locus between the AV and AJ gene segments. It is flanked by TCRD deleting elements (ιJA and δREC) such that rearrangements of the deleting elements to each other or rearrangement of AV to AJ gene segments causes deletion of the intervening TCRD gene locus. The germline encoded TCRD locus consists of eight V segments, three D segments, four J segments, and a single C segment.14 TCRDV2 is located immediately upstream of the D and J segments, whereas TCRDV3 is located downstream of the TCRDJ cluster in an inverted orientation. TCRDV1 is located among the TCRAV genes 360 kb upstream of the TCRDC gene cluster, whereas the remaining five TCRDV genes (described as TCRAV/DV) are also found among the TCRAV genes.14

V(D)J RECOMBINATION

The T lymphocyte has a unique ability to recognise a vast array of non-self antigens and rearrangement of multiple V, D, and J gene segments accounts for the diversity and specificity necessary for this function. During TCR gene rearrangement the V, D (only in TCRB and TCRD chain rearrangement), and J segments are brought together into a continuous V–D–J coding block (fig 2).15 These events are mediated by recombination activating genes, RAG1 and RAG2,26,27 and the enzyme terminal deoxynucleotidyl transferase (TdT).28 The TCR gene segments, as with the immunoglobulin genes, are flanked by recombination signal sequences (RSS), which act as recognition sites for the VDJ recombinase and mark the points at which somatic recombination occurs. The RSS comprises two conserved sequences, a heptamer (CACA/TGTG) separated by 12 or 23 random nucleotides from a consensus nonamer sequence (ACAAAAACC or GGTTTTTGT). Recombination may only take place between gene segments flanked by different spacers,29,30 and this phenomenon is known as the “12–23” rule.31 Therefore, V gene segments flanked 3′ by a 23 bp spacer can recombine with D segments or directly with J segments, both of which are flanked 5′ by a 12 bp spacer. The rearrangement of TCRB and TCRD is a two step process, in which a D gene first recombines with a J gene and then a V gene recombines with the D–J block (fig 2). Because of the absence of D genes in TCRA and TCRG, only V–J recombination occurs in these two chains.

Figure 2

T cell receptor β gene (TCRB) rearrangement. The RAG mediated recombination of the TCRB locus is a two step process in which a diversity (D) gene segment first recombines with a joining (J) segment, followed by the recombination of a variable (V) gene to the DJ block. The rearranged VDJ segment is then spliced post transcriptionally to the C transcript.

“Diversity of the T cell repertoire is not only a consequence of different V, D, J recombinations but also results from nucleotide insertion and deletion at junctional sites”

During these recombination events, diversity at junctional regions is further increased by the incorporation of template dependent palindromic “P” nucleotides,32,33 incorporation of template independent GC rich “N” nucleotides by TdT,34,35 deletion of gene encoded nucleotides by exonucleolytic activity, and imprecise joining of gene segments. The junctional region (V(D)J) encodes the hypervariable CDR3.36 The CDRs (CDR1 and CDR2 within the V genes and CDR3 at the V(D)J junction) are regions of greatest sequence variability, which constitute the binding site for antigen with the CDR3 positioned at the centre of the antigen binding site for direct contact with the MHC bound peptide. Therefore, diversity of the T cell repertoire is not only a consequence of different V, D, J recombinations but also results from nucleotide insertion and deletion at junctional sites, thereby increasing diversity for recognition of a vast array of antigens. Furthermore, diversity is also created by the combinatorial association of individual TCRA and TCRB chains, and TCRG and TCRD chains. It is estimated that 1015 unique TCRs can be produced in this way.31

THE HUMAN T CELL REPERTOIRE

There are several intrinsic and extrinsic factors that influence the expression of the TCR repertoire in humans. These include the human MHC (HLA) system37–39 and TCR gene polymorphisms,25,40–47 in addition to environmental factors, such as infection48–52 and superantigens.53–56 In normal individuals the TCR repertoire is stable and polyclonal. Whereas clonal populations are the hallmark of malignancy, clonal or oligoclonal populations of T and B cells may occur in non-malignant conditions including: human immunodeficiency virus (HIV) and Epstein-Barr virus (EBV) infections with specificity for virus,48,57–59 in the elderly,60,61 in autoimmunity,62–65 in common variable immunodeficiency (CVID),66,67 and in severe combined immunodeficiency (SC1D), where RAG1 or RAG2 gene defects allow only a limited number of successful antigen receptor gene rearrangements, as occurs in patients with the Omenn’s SCID variant.68

ANALYSIS OF TCRVB REPERTOIRE

Phenotype: flow cytometric analyses

Flow cytometry offers a quick, relatively inexpensive method of analysing the TCR repertoire, which is reproducible between laboratories and allows a quantitative evaluation of the expression of different V genes within different T cell subsets—that is, CD4+ and CD8+ cells. Whole blood can be analysed by three/four colour flow cytometry using fluorescently labelled monoclonal antibodies against TCRV family gene segments, together with anti-CD3 and anti-CD4 or anti-CD8 monoclonal antibodies (fig 3). Monoclonal antibodies are available to all the TCR chains for detection of TCRαβ and γδ positive populations. In particular, monoclonal antibodies now cover > 80% of the whole TCRVβ repertoire and reference values of the Vβ repertoire in healthy controls from different age groups are available.69 In contrast, only a few monoclonal antibodies recognise the Vα genes because of the vast number of TCRA V and J recombinations and the polymorphic nature of the α genes. TCRVβ antibody studies can be used as an initial screening method for the detection of expanded families and/or skewing of the T cell repertoire in various disease states, including suspect T cell malignancies and proliferations, and immunodeficiency (fig 3).70

Figure 3

Representative flow cytometric T cell receptor β variable gene (TCRVB) profiles of a healthy control compared with a patient with T cell lymphoma. TCRVβ repertoire analysis was performed using triple colour analysis with CD3 in combination with two different TCRVβ antibodies for each tube. Images on the left show CD3 versus side scatter (SSC) gating to identify the T cells. Images on the right show staining using Vβ2 and Vβ9 labelled antibodies. Only small percentages of Vβ positive T cells can be identified in the healthy control (upper panel), whereas a large T cell population expressing Vβ9 (64%) was identified in the T cell lymphoma (lower panel).

Genotype: PCR methods

The advent of the polymerase chain reaction (PCR) technique has largely replaced Southern blot analysis for clonality studies because PCR offers several advantages, with the principal being that it is quicker to perform and poor quality DNA can be used. The type of PCR approach will depend on whether DNA or RNA based methods are applicable. Paraffin wax embedded material is often the only material available in routine diagnostic pathology laboratories, so protocols are restricted to DNA based methods and the amplification of small (< 250 bp) PCR fragments.71 Both DNA and RNA methods are suitable for fresh material.

Reverse transcription PCR (RT-PCR) using RNA can be used for the analysis of the expressed TCR repertoire. Suitable PCR methods include: (1) amplification of all V segments using family specific primers complementary to all TCRV genes and a constant region primer70,72 (although easy and reliable, this method at best can only provide a semiquantitative profile of TCRV usage); (2) modification of all TCR transcripts so that they can be amplified by one oligonucleotide (anchor PCR)73; and (3) the production of circular transcripts by ligation (inverse PCR).74 These last two methods are technically demanding but allow quantitative analysis of the T cell population. Such analyses have been applied to clonality studies and the evaluation of the TCR repertoire in immunodeficiency, autoimmune disease, and alloreactive T cells in allografts.75

Methods for routine clonality assessment should be simple and robust with good detection rates and sensitivity rates of ∼ 5%. Although clonality assessment can be completed using RNA and RT-PCR, analysis of DNA is more common and applicable to poor quality DNA extracted from paraffin wax embedded, formalin fixed tissue. Many methods applicable to DNA rely on family consensus primers and therefore suffer from false negativity as a result of improper annealing of PCR primers to all gene segments because of the lack of homology of the primers to template DNA. Furthermore, some approaches have used nested PCR, which may suffer from contamination problems. Presently, a European project (BIOMED-2 concerted action (PL-96–3936)) is trying to deal with the problem of false negatives common to all PCR protocols. The project has involved the design of new and specific primers for all TCR and immunoglobulin (Ig) loci and the standardised testing of all cases. This approach has already increased detection rates and sensitivity.

PCR analysis of the TCRG genes is the protocol mostly widely applied to clonality studies in clinical pathology laboratories because of the restricted germline repertoire of the TCRG genes, which limits the number of required PCR primers.76 However, the limited repertoire also results in high background amplification of similar rearrangements in normal T cells. Furthermore, TCRG gene rearrangements can be used as markers of clonality, not only in TCRγδ positive malignancies (where the rearrangements are functional) but also in TCRαβ positive malignancies, where non-functional TCRG gene rearrangements are seen as a consequence of the ordered hierarchy of TCR loci gene rearrangements.

Analysis of TCRD genes is mainly restricted to TCRγδ positive malignancies, which are fairly rare, because the TCRD genes are deleted in most TCRαβ positive T cell malignancies. However, the TCRD locus is extensively used as a target in minimal residual disease (MRD) detection in both T and B lineage acute lymphoblastic leukaemia (ALL), particularly because cross lineage TCRD gene rearrangements occur extensively in precursor B cell ALL (B-ALL).77 The TCRD is a useful target as a result of the combination of three factors, namely: (1) limited germline repertoires, (2) specific recombination patterns in different subtypes of ALL, and (3) extensive junctional diversity.78

“PCR analysis of the TCRG genes is the protocol mostly widely applied to clonality studies in clinical pathology laboratories because of the restricted germline repertoire of the TCRG genes, which limits the number of required PCR primers”

The TCRB locus is the locus of choice for establishing clonality of TCRαβ expressing tumours, which comprise more than 90% of lymphoid malignancies. DNA based methods have exploited the extensive TCRB germline repertoire with varied success.71,79 However, recently family specific BV primers have been applied to DNA as part of the BIOMED-2 programme and have achieved improved detection rates for TCRB clonality. The TCRA locus has proved too complex for suitable DNA clonal analyses.

Detection systems

TCR PCR fragments may be detected in a variety of ways to discriminate the junctional diversity between polyclonal and clonal populations. These methods include fluorescent gene scanning,80,81 heteroduplex analysis,82–84 single strand conformation polymorphism (SSCP),85 denaturing gradient gel electrophoresis (DGGE),86,87 cloning, and sequencing.88

Fluorescent gene scanning involves the amplification of specific TCR genes using fluorescently labelled primers. Such analysis is used in routine clonality assessment of TCRG and TCRB genes, TCRBV family usage, and MRD studies. The resulting PCR products are run on a gene sequencer and analysed using GeneScan software (Applied Biosystems, Warrington, Cheshire, UK). Polyclonal populations with varying PCR fragment lengths demonstrate a normal Gaussian distribution visualised as a series of peaks. Clonal populations are seen as distinct peaks (fig 4).

Figure 4

Heteroduplex and fluorescent gene scanning analyses. The same T cell receptor β gene (TCRB) polymerase chain reaction (PCR) products, amplified using the BIOMED TCR VB–JB primers (JJM van Dongen et al, unpublished data) are shown by (A) heteroduplex analysis and (B) fluorescent gene scanning. (A) M, molecular weight size marker, with sizes indicated in base pairs; P, polyclonal peripheral blood control; C, clonal T cell lymphoma control. In the polyclonal control, only heteroduplexes are visible as a smear at ∼ 500–550 bp, which results from the re-annealing of the polyclonal PCR fragments of different sizes and sequences. The clonal homoduplex PCR product is indicated by an arrow at about ∼ 259 bp, whereas the heteroduplex PCR products in the sample run behind at about 500–550 bp. (B) Relative fluorescent intensities (ordinate) are plotted as a function of PCR fragment size (abscissa). The polyclonal control in the upper panel shows a normal Gaussian distribution of PCR products of different sizes within the appropriate size range (250–290 bp). The clonal T cell lymphoma control in the lower panel shows a dominant fluorescent peak indicative of a clonal population with identical PCR fragment sizes at ∼ 259 bp.

Heteroduplex analysis (and the less frequently used SSCP analysis), rely on conformational changes caused by mismatch at the junctional region visualised by retardation on a gel matrix. True clonal PCR products give rise to homoduplexes, whereas polyclonal PCR products form heteroduplexes upon renaturation. Because of differences in conformation, the clonal homoduplexes (PCR products with identical junctional regions from clonal populations) migrate more rapidly through the gel and are seen as intense clonal bands, whereas heteroduplexes with less perfectly matched sequences (PCR products with heterogeneous junctional regions) appear as ill defined smears running behind the homoduplex (fig 4). DGGE allows rapid screening for single base changes at the CDR3 region. This technique is based on the migration of double stranded DNA through a polyacrylamide gel containing increasing concentrations of a denaturing agent.89

Cloning and sequencing provides perhaps the best method of assessing clonality within T cell populations, allowing a detailed analysis of the CDR3 region, including V, D, and J gene identification (using internet databases such as www.ncbi.nim.nih.gov/BLAST and http://imgt.cines.fr.8104), in addition to the identification of non-template encoded nucleotides; however, this technique is both costly and labour intensive.

Cross lineage rearrangements

It was originally hoped that clonal gene rearrangements of TCR and Ig loci would accurately assign T or B cell lineages to clonal lymphoid proliferations, respectively; however, it was soon evident that cross lineage rearrangements—that is, TCR and IgH gene rearrangements in B and T cell lymphoid neoplasms, respectively—occurred.90 Both Ig and TCR genes are flanked by RSS and therefore inappropriate gene rearrangements reflect gene segments that are accessible for recombination, but which do not reflect the final lineage commitment of the tumour cells. Approximately 25–30% of high grade lymphoid tumours and 5–10% of low grade tumours have cross lineage rearrangements: partial rearrangements (D–J recombinations) occur more frequently than complete (VDJ or VJ) rearrangements. Furthermore, cross lineage rearrangements are more common among immature lymphoid neoplasms (ALL) than mature neoplasms.77,90 Cross lineage Ig gene rearrangements are usually restricted to the Ig heavy chain gene in T cell neoplasia; Ig light chain genes have rarely been found.

THE DIAGNOSTIC ROLE OF TCR GENE REARRANGEMENT STUDIES

T cell lymphomas and leukaemias

The molecular diagnosis of non-Hodgkins lymphoma and leukaemia is based on the fact that all cells have a common clonal origin and > 98% of cases exhibit clonal rearrangements of TCR and Ig genes, reflecting their cellular origins from T or B cells, respectively.77 Molecular analyses are extremely helpful for diagnosis in cases with unusual morphological features, confusing immunophenotypic characteristics, or where a phenotypic clonal marker is lacking. Nevertheless, it should be remembered that the presence of clonal gene rearrangements does not necessarily imply malignancy because several benign or reactive inflammatory conditions may show such a pattern. Some examples include benign inflammatory disorders of skin, such as lymphomatoid papulosis and pityriasis lichenoides et varioliformis acuta, where clonal T cell expansions are evident.91–93 Conversely, the absence of a clonal gene rearrangement does not rule out malignancy because the population of malignant cells may be at a level below the sensitivity of the technique. The current consensus view is that lymphomas can only be defined by a combination of clinical, histological, and immunological data.94

Table 2 shows the incidence of clonal rearrangements in various lymphoproliferative disorders. At our centre, 60% of all lymphoproliferations referred for clonal investigations are suspect T cell lymphoproliferations, and more than 65% of these specimens are referred as formalin fixed, paraffin wax embedded biopsies. Two problematical areas of diagnosis include lymphoproliferations in skin and peripheral T cell lymphoma (PTCL).

Table 2

Incidence of clonal gene rearrangements in T and B cell lymphomas and leukaemias

More than 25% of referrals to our laboratory are skin lymphoproliferations. The diagnosis of lymphoproliferations in skin is a difficult task, because lymphoid infiltrates with similar histological features may represent widely disparate clinical diseases. Conversely, the histological picture associated with certain diagnoses such as mycosis fungoides can vary greatly between individuals and at different times in the clinical course of the disease. TCR and clonality studies have proved to be of immense help in the diagnosis and follow up of patients with cutaneous T cell lymphomas.91

The interpretation of clonality in PTCL can also be difficult, especially those of the angioimmunoblastic type (AILD), where the interpretation of clonality is complicated by the heterogeneity of TCR and IgH rearrangements. In addition, clonal rearrangements have been shown to regress or appear during the course of disease, suggesting that these cases harbour multiple clones similar to those found in cases with acquired immunosuppression. Although most cases exhibit TCR rearrangements, we have recently shown that functional T and B cell oligoclones can be found in up to 30% of cases.96 Our data do not support the concept of AILD as a clearly defined PTCL, but offer experimental evidence in support of the observation of Lipford et al,97 who concluded that AILD is a disease of proliferating lymphoid clones in which supervening malignant lymphomas may develop by a process of clonal selection. Figure 4 shows a malignant T cell proliferation as detected by heteroduplex analysis and fluorescent gene scanning.

“The presence of clonal gene rearrangements does not necessarily imply malignancy because several benign or reactive inflammatory conditions may show such a pattern”

During the treatment of many haematological malignancies, tumour cells may become undetectable in blood, bone marrow, etc by conventional means. However, many cases may still relapse, indicating that biological remission has not been achieved. MRD describes the presence of tumour cells at a level below that detectable by conventional methodology and provides accurate information about the efficacy of treatment regimens.77,98 Furthermore, MRD analysis can predict outcome by determining malignant cell burden during initial treatment. Most MRD assays use “real time” technology to quantitate residual disease. Such assays exploit the 5′→3′ nuclease activity of Taq polymerase to detect and quantify specific PCR products.99,100 Using this technique, the PCR product is measured at each cycle by cleavage of a fluorogenic intrinsic probe by the emission of a fluorescent signal that accumulates during the reaction. Because post-PCR processing is not necessary, quantitative data can be accumulated quickly. Real time primers and probes have been applied to patient specific junctional regions in ALL, in addition to BCR–ABL fusion transcripts in chronic myeloid leukaemia, to provide accurate and reliable data for MRD detection.101,102

Large granular lymphocytoses

The syndrome of large granular lymphocytosis (LGL) with chronic neutropenia was recognised as a distinct clinical entity in 1977. Two types of LGLs have been described on the basis of their lineages: CD3+ LGL and CD3− LGL. Whereas CD3+ LGLs express and rearrange TCRs, CD3− LGLs are composed of natural killer cells which do not rearrange their TCR or Ig genes.103,104 Although clonality can be easily demonstrated in the more common CD3+ LGL by the detection of TCR gene rearrangement, clonality is less easy to demonstrate in CD3− LGL, although clonal analysis using the lineage independent method of X inactivation has been applied to this group.105 Furthermore, the detection of a single episomal form of EBV has been used as evidence that the LGL has arisen from a single EBV infected cell.106

One of the key questions in this disorder has been whether LGL represents a “reactive” or “neoplastic” proliferation. It should also be noted that oligoclonal/clonal T cell expansions are frequently seen in the peripheral blood of normal healthy individuals. Such expansions are most prominent among CD8+ T cells and are increasingly present with age, being detectable in adults (> 35 years of age) and becoming very frequent in the elderly (> 70 years of age).60,107–109 However, such populations are not unknown in the young. We have recently described two paediatric cases presenting in the 2nd decade of life with neutropenia and sepsis. Both cases had expanded clonal populations of TCRBV13 CD8+ T cells. Both patients received cyclosporin and showed pronounced clinical improvement. In contrast, the CD4+ T cell repertoire remains largely polyclonal throughout life, although expanded CD4+/CD45RO+ populations have been detected in elderly individuals.61

Although some reports have highlighted that clonality studies and CD8 TCRVB family profiling are useful in monitoring disease progression and response to treatment in LGL, it has been suggested that the morbidity and mortality in LGL is primarily related to neutropenia rather than lymphocytosis.104 Most of our current knowledge regarding the management of these patients comes from anecdotal case reports and with the uncertainty in the diagnosis, the indication for beginning aggressive immunosuppressive treatment remains unclear.

Infection

Infections, particularly viral (EBV, cytomegalovirus (CMV), human T cell lymphotropic virus (HTLV), and HIV) and staphylococcal toxic shock syndrome, are known to induce lymphocytosis, usually polyclonal, but occasionally clonal.110–112 The lymphocytoses in CMV, EBV, and HTLV infection are usually associated with an expansion of LGLs. After EBV infection, memory clonotypic CD8+ cells persist at relatively high circulating frequencies and are necessary for maintaining a balanced host–virus relationship in normal individuals. More recently, oligoclonality has also been seen in hepatitis B virus specific CD8+ cells using HLA-A2 peptide tetramers.113

EBV is commonly associated with post transplant lymphoproliferative disease of oligoclonal B cell origin. T cell proliferations after transplantation are uncommon but such populations have been reported.114 Reactive oligoclonal populations can also occur following transplantation that represent re-population of the bone marrow, and which cannot be interpreted as re-occurrence of malignancy without reference to the original clone.

TCR family expansions have also been reported in HIV positive patients with low CD4+ cell counts, and it has been suggested that the virus per se may have a superantigen effect on the TCR.115,116 Recent studies have also shown that in the early phases of HIV infection there is a transient expansion of CD8+ T cells, which in some patients may persist for six to 30 months.117 CD8+ T cells play an important role in protection and control of HIV-1 by direct cytolysis of infected cells and by suppression of viral replication by secreting cytokines, chemokines, and CD8+ T cell antiviral factors. Although clonality and oligoclonality can be demonstrated in a proportion of these cells they represent a reactive expansion of CD8+ T cells rather than malignancy. A decline in reactive clonal CD8 cells has been linked to progression to AIDS, with a strong inverse correlation between the in vivo frequency of HIV specific clones and levels of viraemia.118

Immunodeficiency

In many primary immunodeficiency disorders a high degree of clonality/oligoclonality is present within lymphocyte populations. Restricted heterogeneity of T cell subpopulations has been described in Omenn’s syndrome, with skewing of the TCRAB repertoire and the presence of oligoclonal T cell populations.68,119 Omenn’s syndrome was first described in 1965 as a severe form of SCID, characterised by erythroderma, failure to thrive, and lymphadenopathy. High numbers of activated, anergic T cells are usually present and infiltrate skin and gut epithelium. Recent reports have described mutations in RAG1 and RAG2 genes in this syndrome.120,121 However, clinically defined Omenn’s syndrome may be a more complex entity than is currently recognised.120 We have recently studied eight children with clinically defined Omenn’s syndrome and two of the eight demonstrated RAG gene mutations. Alterations of the TCRVB repertoire were seen with five of the eight children demonstrating oligoclonal TCRV gene rearrangements. Although failure to demonstrate TCRVB clonality may arise from the lack of homology of V primers to all known V genes, these results suggest that TCRVB skewing and clonality may not be seen in all cases and may, in part, be related to the type of RAG gene mutation.123 Furthermore, oligoclonal B cell populations found in one child in the absence of clonal T cells were confirmed by gene sequencing (J L Smith et al, unpublished data, 2002). Such data suggest that Omenn’s syndrome is a more heterogeneous disease than previously recognised.

The differentiation of materno–fetal graft versus host disease from Omenn’s syndrome in patients with SCID and similar presenting features can be resolved by chimaerism studies. Although materno–foetal grafts have not been reported to show the alterations in the T cell repertoire commonly seen in patients with Omenn’s syndrome, studies for TCRG/TCRD clonality analysis may be useful. We have identified three cases of materno–fetal engraftment with clonal TCRGD populations in the blood (J L Smith et al, unpublished data, 2002).

Take home messages

  • T cell receptor (TCR) gene rearrangement is an important event in T cell ontogeny that enables T cells to recognise antigens specifically, and any dysregulation in this process may result in disease

  • Techniques such as Southern blot hybridisation, polymerase chain reaction (PCR), and flow cytometry have enabled T cell proliferations to be characterised in malignancy and in diseases where T cells have been implicated in the pathogenesis, such as immunodeficiency, autoimmunity, and atopy

  • One of the most important of these applications is the diagnosis of lymphocytic disease and its differentiation from reactive lymphoproliferations

  • These applications are less well defined for patient monitoring but are useful for monitoring minimal residual disease (MRD) and in patients with CD8+ lymphocytoses

  • Methods based on real time PCR are also available for monitoring MRD after treatment or bone marrow transplantation

  • TCR gene studies have been diagnostically useful for the definition of immunodeficiency states, most notably, Omenn’s syndrome, and for the characterisation of T cells at disease sites in patients with autoimmune disease or allergy

  • However, PCR technology has a high incidence of false negativity, resulting from the use of consensus primers, so that several groups are working towards the design of better primers

Clonal or oligoclonal lymphocyte populations can also be found in other children with primary immunodeficiency. In a study of genomic DNA, extracted from blood lymphocytes from 86 children with defined and undefined immunodeficiency, and using heteroduplex analysis we have found clonal or oligoclonal populations of T and/or B cells in 37 cases. Of these 37 children, 84% had clonal TCR gene rearrangements and 35% had clonal IgH rearrangements. In a control study of 26 children matched for age and sex we did not find clonal/oligoclonal populations (J L Smith, et al unpublished data, 2002). The origin of these clonal populations is uncertain; however, from the diversity of the patient population studied it is clear that intrinsic factors alone cannot explain these results and that extrinsic factors may have an important role.121

“Although clonal rearrangements are the hallmark of lymphoid tumours, monoclonal proliferations have been reported in patients with CVID without progression”

The most common type of primary immunodeficiency in clinical practice is CVID.124 Significant differences in TCRBV gene usage has been reported in patients with CVID, and in particular those with an associated CD8+ lymphocytosis.124,125 Up to 15% of patients with CVID have lymphoadenopathy, with granulomatous lymphadenopathy occurring in 75% of all biopsies.126 The remainder, including reactive and atypical lymphoid hyperplasia, lack distinctive features and in these patients prediction of clinical outcome is highly unsatisfactory.66 Lymphomas occurring in patients with CVID are usually diffuse and are of intermediate or high grade in the working formulation. The diagnosis is complicated by the fact that lymphoid tissue from all patients with CVID can be histologically abnormal because germinal centres are frequently absent or poorly developed, so that stimulation by infection can result in histologies that are difficult to differentiate from lymphoma.127

Lymphomas of B cell type predominate in CVID, although T cell types have been reported128 The interpretation of clonality in this group of patients is complex. Although clonal rearrangements are the hallmark of lymphoid tumours, monoclonal proliferations have been reported in patients without progression. In a recent UK study, we have reviewed clonality in biopsies of reactive and lymphoma histologies. Although clearly present in lymphoma tissue, clonal populations of B cells were also found in reactive biopsies and biopsies of equivocal lymphoma histology. Apparent clonality may result from small numbers of B cells present in some biopsies, but this was not true in all biopsies of reactive histology. In CVID, clonal populations cannot be definitive for lymphoma and these findings must be interpreted with the immunohistology of the biopsy and the clinical presentation of the patient.

Clonality in autoimmunity and atopy

In many autoimmune and atopic disorders localised T cell expansions have been described in the affected tissue and subsequent clonality studies have identified increased oligoclonality within T cells at these sites. In both rheumatoid arthritis and multiple sclerosis, various different TCRAV and TCRBV segments have been found to be expanded, and oligoclonal/clonal populations of CD4+ and CD8+ T cells have been identified at the disease sites.62,129–133; however, no consistent usage of TCRBV families has been identified between studies. Some of these studies have implications for immunotherapy.134

Clonal and oligoclonal lymphocyte expansions can be found in the lungs of atopic and non-atopic patients with asthma and normal individuals.135 Therefore, it is difficult to comment on the clinical relevance of these findings; however, clonal populations can also be found after allergen challenge, suggesting a specific T cell response in some cases. Although the lung T cell repertoire is broadly representative of blood T cells, there does appear to be selection and amplification of specific T cell families in the lung in response to local antigenic stimulation.136 In a recent study, we examined the clonality of the T cell response to peanut in MHC class II identical peanut allergy discordant siblings. In vitro stimulation of peripheral blood mononuclear cells with crude peanut extract resulted in stimulation of clonal/oligoclonal T cell responses, together with increased usage of TCRVB11 in peanut allergic individuals.137

Analysis of clonality is a further means of characterising T cells in clinical hypersensitivity reactions; however, apparent specific T cell responses to antigen in some atopic individuals, characterised by oligoclonality of T cell populations at the affected site, do not always provide unique clonality profiles that could be used as diagnostic markers for these diseases.

CONCLUSION

Advances in molecular biological techniques have provided a variety of methods for the investigation of disease. Methods are applicable to RNA and DNA, including poor quality DNA isolated from paraffin wax embedded biopsy material, thus enabling retrospective analyses to be carried out. Foremost among these applications is the diagnosis of lymphocytic disease and differentiation from reactive lymphoproliferations. These applications are less well defined for patient monitoring but are useful for monitoring MRD and in patients with CD8+ lymphocytoses. Methods based on real time PCR are also available for monitoring residual disease after treatment or bone marrow transplantation. The study of the T cell repertoire has proved diagnostically useful for the definition of immunodeficiency states, most notably, Omenn’s syndrome, and for the characterisation of T cells at disease sites in patients with autoimmune disease or allergy. Nevertheless, one of the limitations of PCR technology is the high incidence of false negativity, resulting from the use of consensus primers. Currently, there are several groups working towards the design of better primers,38 which we hope will be available in forthcoming years.

REFERENCES

View Abstract

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.