J Clin Pathol 58:734-739 doi:10.1136/jcp.2004.023531
  • Original article

Cell cycle related proteins as prognostic parameters in radically resected non-small cell lung cancer

  1. V Esposito1,
  2. A Baldi2,
  3. A De Luca3,
  4. G Tonini4,
  5. B Vincenzi4,
  6. D Santini4,
  7. P Persichetti5,
  8. A Mancini6,
  9. G Citro7,
  10. F Baldi2,
  11. A M Groeger1,
  12. M Caputi6
  1. 1International Society for the Study of Comparative Oncology (ISSCO), Silver Spring, MD 209 06, USA
  2. 2Department of Biochemistry and Biophysics “F. Cedrangolo”, Section of Anatomic Pathology, Second University of Naples, Naples 80138, Italy
  3. 3Department of Medicine and Public Health, Section of Clinical Anatomy, Second University of Naples
  4. 4Section of Oncology, Campus BioMedico University, Rome 00100, Italy
  5. 5Section of Plastic and Reconstructive Surgery, Campus BioMedico University
  6. 6Department of Cardiological, Respiratory and Thoracic Medical Sciences, Second University of Naples
  7. 7SAFU Department, Regina Elena Cancer Institute, Rome 00100, Italy
  1. Correspondence to:
 Dr A Baldi
 Via G. Orsi 25, 80128 Naples, Italy;
  • Accepted 4 January 2005


Background: Experimental evidence suggests that lung cancer development and progression can be linked to an increased proliferation rate.

Aims/Methods: To evaluate the immunohistochemical expression of seven components of the cell cycle machinery in a series of well characterised non-small cell lung cancer (NSCLC) specimens (n  =  105).

Results: Multivariate analysis revealed that simultaneous loss of expression of three of these factors—cyclin D1, the cyclin dependent kinase inhibitor p16, and the tumour suppressor retinoblastoma protein Rb2/p130—correlated with survival, confirming the hypothesis that the cyclin D1–p16–retinoblastoma tumour suppressor pathway is inactivated in most lung cancer samples.

Conclusions: These results suggest that loss of control of cell cycle checkpoints is a common occurrence in lung cancer and support the idea that functional cooperation between different cell cycle regulatory proteins constitutes another level of regulation in cell growth control and tumour suppression.