Article Text
PDFShort report
Direct polymerase chain reaction amplification of formalin-fixed, paraffin-wax-embedded tissue after automated sequential laser microdissection and pressure catapulting
Abstract
A robust method to facilitate rapid laser microdissection and pressure catapulting (LMPC) coupled with direct polymerase chain reaction (dPCR) to eliminate the need for extraction of DNA before a PCR-based assay is described. This sequential LMPC–dPCR method is rapid and decreases the number of processing steps, reducing the chance of tissue loss and contamination.
- dPCR, direct polymerase chain reaction
- FFPE, formalin-fixed, paraffin-wax-embedded
- LMPC, laser microdissection and pressure catapulting
- PCR, polymerase chain reaction
- PEN, polyethylene naphtahalate
- sLMPC, sequential laser microdissection and pressure catapulting