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Prognostic significance of p21WAF1/CIP1, p27Kip1, p53 and E-cadherin expression in gastric cancer
  1. Armando Gamboa-Dominguez1,
  2. Stefan Seidl2,
  3. Edgardo Reyes-Gutierrez1,
  4. Christine Hermannstädter2,
  5. Leticia Quintanilla-Martinez3,
  6. Raymonde Busch4,
  7. Heinz Höfler2,
  8. Falko Fend2,
  9. Birgit Luber2
  1. 1Department of Pathology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Mexico City, Mexico
  2. 2Technische Universität München, Klinikum rechts der Isar, Institut für Allgemeine Pathologie und Pathologische Anatomie, München, Germany
  3. 3GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Pathologie, Neuherberg, Germany
  4. 4Technische Universität München, Klinikum rechts der Isar, Institut für Medizinische Statistik und Epidemiologie, München, Germany
  1. Correspondence to:
 Dr Birgit Luber
 Institut für Allgemeine Pathologie und Pathologische Anatomie, Trogerstr. 18, 81675 München, Germany; luber{at}lrz.tu-muenchen.de

Abstract

Background: Gastric carcinoma is characterised by numerous genetic and epigenetic alterations that influence cell cycle progression, apoptosis and DNA repair. These alterations include down-regulation of the cyclin-dependent kinase (CDK) inhibitors p21WAF1/CIP1 and p27Kip1, and mutations of the tumour suppressor protein p53 and the cell adhesion molecule E-cadherin. Combined evaluation of the prognostic significance of these alterations has not been reported in Mexican Mestizo patients.

Aims: To evaluate p21WAF1/CIP1, p27Kip1, p53 and E-cadherin protein expression, including mutant E-cadherin variants with deletion of exon 8 (del 8) or 9 (del 9), in gastric cancer from Mexican patients.

Methods: Immunohistochemistry for the above-mentioned markers, including mutation-specific E-cadherin antibodies, was carried out in 69 gastric carcinomas; expression levels were correlated with histotype, tumour stage and prognosis.

Results: Expression of p21WAF1/CIP1 alone or in combination with p27Kip1 or in the absence of p53 was associated with favourable prognosis. Staining of del 8 and del 9 E-cadherin was found exclusively in patients negative for p53 and positive for p21WAF1/CIP1, suggesting that the p21WAF1/CIP1 regulatory function of p53 was intact.

Conclusion: Combined evaluation of the prognostic significance of cell cycle regulators and E-cadherin should be performed. Even though patients negative for p53 and positive for p21WAF1/CIP1 have a favourable prognosis, it may have a negative influence on prognosis if they acquire in addition E-cadherin mutations which have been shown previously to be associated with poor survival.

  • CDK, cyclin dependent kinase
  • del 8 E-cadherin, E-cadherin with deletion of exon 8
  • del 9 E-cadherin, E-cadherin with deletion of exon 9
  • TNM, tumour node metastasis
  • p21WAF1/CIP1
  • p27Kip1
  • p53
  • E-cadherin
  • gastric cancer

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Footnotes

  • Published Online First 4 May 2007

  • Funding: The study was supported by a grant to Drs B Luber and I Becker from the Wilhelm-Sander-Stiftung (Nr 1999.118.2).

  • Competing interests: None.

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