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Human herpes virus 6 in archival cardiac tissues from children with idiopathic dilated cardiomyopathy or congenital heart disease
  1. M Comar1,4,
  2. P D’Agaro1,
  3. C Campello1,
  4. A Poli2,
  5. J P Breinholt III3,
  6. J A Towbin3,
  7. M Vatta3
  1. 1Department of Public Medicine Sciences, UCO Hygiene and Preventive Medicine, University of Trieste, and Institute of Child Health IRCCS Burlo Garofolo, Trieste, Italy
  2. 2Department of Medicine and Public Health, University of Verona, Italy
  3. 3Pediatric Cardiology, Baylor College of Medicine and Texas Children’s Hospital, Houston, Texas, USA
  4. 4Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA
  1. Professor C Campello, Department of Public Medicine Sciences, University of Trieste, Via dell’Istria 65/1, 34137 Trieste, Italy; campello{at}burlo.trieste.it

Abstract

Objective: To explore the possible role of human herpes virus 6 (HHV-6) in cardiac disorders in childhood in a retrospective study on archival specimens of explanted hearts.

Methods: 16 children (median age at transplantation 11.0 years) with idiopathic dilated cardiomyopathy (DCM) and 19 children (median age at transplantation 1.0 year) with congenital heart disease (CHD), previously found to be negative for other cardiotropic viruses such as enteroviruses, adenovirus, parvovirus B19, cytomegalovirus and Epstein–Barr virus, were tested for HHV-6 by quantitative real-time PCR and by genotyping. In addition, HHV-7/8 infection was investigated by qualitative PCR.

Results: HHV-6 B variant was detected in 11 of 35 samples (31.4%) with a mean viral load of 3.1×102 copies/μg of DNA. When assessed by heart disorder, the prevalence was different in the two groups (43.7% in DCM and 21% in CHD) while the mean viral loads were similar. In a logistic multivariate analysis HHV-6 was independently associated with DCM, taking CHD as reference and adjusting for age (best estimate: OR = 6.94; 95% CI 1.00 to 49.85; p = 0.05).

Conclusions: Although the clinical significance of the results is unknown, HHV-6 B genome is frequently detected in explanted hearts from children with DCM and to a lesser extent with CHD, thus adding evidence for HHV-6 cardiac involvement.

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Footnotes

  • Funding: MV is funded by the NIH (grant numbers HL077706 and HL078807). MC is funded by grant R.C. 70/2005 from the IRCCS Burlo Garofolo, Trieste, Italy. JAT is funded by the NIH (grant numbers HL67155 and HL65652), the Abby Glaser Fund, the Vivian L Smith Foundation, and the Children’s Cardiomyopathy Foundation.

  • Competing interests: None.

  • Ethics approval: Ethics approval was obtained.

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