Article Text

PDF
Validation of tissue microarray technology in malignant peripheral nerve sheath tumours
  1. K Soares Gonçalves Cunha1,2,3,
  2. A Cunha Caruso4,
  3. A Soares Gonçalves1,
  4. V Gonçalves Bernardo1,
  5. A Rodrigues Cordovil Pires1,
  6. E Carvalho da Fonseca1,
  7. P Antônio Silvestre de Faria4,
  8. L Esmeraldo da Silva1,
  9. M Geller3,5,6,
  10. R Soares de Moura-Neto7,
  11. V Silami Lopes1
  1. 1
    Postgraduate Program of Pathology, School of Medicine, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil
  2. 2
    School of Dentistry, Nova Friburgo University Pole, Fluminense Federal, University (UFF), Nova Friburgo, Rio de Janeiro, Brazil
  3. 3
    Postgraduate Program of Medicine, Carlos Chagas Institute, Rio de Janeiro, Brazil
  4. 4
    Pathology Division, National Institute of Cancer (INCA), Rio de Janeiro, Brazil
  5. 5
    Department of Microbiology and Immunology, Teresópolis Medical School (UNIFESO), Teresópolis, Rio de Janeiro, Brazil
  6. 6
    Department of Clinical Genetics, Martagão Gesteira Institute of Puericulture and Pediatrics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil
  7. 7
    Department of Genetics, Biology Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil
  1. Dr K Soares Gonçalves Cunha, Rua Marquês do Paraná, 303 - 4° andar - sala 1 - Centro, Niterói, Rio de Janeiro, Brazil 24033-900; karingoncalves{at}terra.com.br

Abstract

Background: It has been suggested that the donor tissue cores used in tissue microarrays (TMAs) may not be representative of the whole tissue section.

Aim: To validate the use of TMA technology in the study of malignant peripheral nerve sheath tumours (MPNSTs).

Methods: A TMA was constructed containing five independent core biopsy samples of 14 formalin-fixed, paraffin-embedded MPNSTs. The immunohistochemical (IHC) results of the five cores from the same tissue block on TMA were compared with readings from whole sections using two antibodies: anti-Ki-67 and anti-S-100. Digital image analysis was performed to calculate the percentage of positive stain areas. The agreement between IHC results obtained with TMA cores and whole sections was assessed using the κ statistic.

Results: There was good to very good agreement between IHC results for whole and TMA sections from MPNSTs. In relation to S-100, very good agreement (92% agreement; κ = 0.77) was observed using a minimum of four TMA cores. Staining results for Ki-67 from at least four readable TMA cores were the same as those for the whole section in 86% of cases, with good agreement, using weighted κ statistics (κ = 0.63).

Conclusions: This study indicates that the TMA technique can be used in the IHC study of MPNSTs, even with the use of heterogeneous markers such as S-100 protein.

Statistics from Altmetric.com

Footnotes

  • Competing interests: None.

  • Ethics approval: Obtained.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.