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Assessment of malignant potential of oral submucous fibrosis through evaluation of p63, E-cadherin and CD105 expression
  1. Raunak Kumar Das1,
  2. Mousumi Pal2,
  3. Ananya Barui1,
  4. Ranjan Rashmi Paul2,
  5. Chandan Chakraborty1,
  6. Ajoy Kumar Ray3,
  7. Sanghamitra Sengupta4,
  8. Jyotirmoy Chatterjee1
  1. 1School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India
  2. 2Guru Nanak Institute of Dental Science and Research, Kolkata, West Bengal, India
  3. 3Electronics & Electrical Communication Engineering, Indian Institute of Technology Kharagpur, West Bengal, India
  4. 4Department of Biochemistry, University of Calcutta, Kolkata, West Bengal, India
  1. Correspondence to Raunak Kumar Das, School of Medical Science & Technology, Indian Institute of Technology, Kharagpur, West Bengal 721302, India; raunak81{at}gmail.com

Abstract

Background The assessment of malignant potential of oral submucous fibrosis grades vis-à-vis their progression towards malignancy is associated with expression of possible multiple molecular markers.

Aims To analyse p63, E-cadherin and CD105 expression in this premalignant pathosis with a view to unravel and understand the expression of these molecules as markers.

Methods The oral mucosal biopsies (normal, oral submucous fibrosis with and without dysplasia) were studied with routine H&E, and by immunohistochemistry for p63, E-cadherin and CD105 expression. p63 was assessed as percentage of positive nuclei. E-cadherin expression was estimated through (i) distance between basement membrane and E-cadherin expression initiation point, (ii) ratio between epithelial thickness and epithelial thickness displaying E-cadherin, and (iii) E-cadherin intensity variation along the expression path. CD105 expression was assessed qualitatively.

Results The p63+ cells were highest in severely dysplastic tissues followed by other dysplastic grades, normal oral mucosa and non-dysplastic conditions. However, the p63+ cells displayed the feature of progressive maturation only in normal mucosa. There was a loss of membranous E-cadherin in basal layers of all diseased conditions; it was highest in severe dysplasia. There was significant variation (p<0.0001) in E-cadherin intensity within and between the tissues (normal and diseased). CD105 expression increased abruptly in dysplasia.

Conclusions The malignant potential of this pre-cancerous condition is likely to be correlated with an increase in p63 and CD105 expression and a concomitant loss of membranous E-cadherin. This may lead to marker identification through greater validation.

  • Oral submucous fibrosis
  • dysplasia
  • p63
  • E-cadherin
  • CD105
  • immunohistochemistry
  • molecular pathology
  • oral pathology

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Footnotes

  • Funding School of Medical Science & Technology, Indian Institute of Technology Kharagpur, West Bengal, India.

  • Competing interests None declared.

  • Patient consent Obtained.

  • Ethics approval This study was conducted with the approval of the Guru Nanak Institute of Dental Science and Research (GNIDSR), Kolkata (GNIDSR/IEC/07/15).

  • Provenance and peer review Not commissioned; externally peer reviewed.

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