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The molecular basis of the chemosensitivity of metastatic cutaneous melanoma to chemotherapy
  1. Katharine A Parker1,
  2. Sharon Glaysher1,
  3. Marta Polak1,
  4. Francis G Gabriel1,
  5. Penny Johnson1,
  6. Louise A Knight1,
  7. Matthew Poole2,
  8. Ajit Narayanan3,
  9. Jeremy Hurren4,
  10. Ian A Cree1
  1. 1Translational Oncology Research Centre, Queen Alexandra Hospital, Portsmouth, UK
  2. 2School of Computing, University of Portsmouth, Portsmouth, UK
  3. 3School of Computing and Mathematical Sciences, Auckland University of Technology, Auckland, New Zealand
  4. 4Department of Surgery, Queen Alexandra Hospital, Portsmouth, UK
  1. Correspondence to Professor Ian A Cree, Translational Oncology Research Centre, Queen Alexandra Hospital, Portsmouth PO6 3LY, UK; ian.cree{at}porthosp.nhs.uk

Abstract

Background Chemotherapy benefits relatively few patients with cutaneous melanoma. The assessment of tumour chemosensitivity by the ATP-based tumour chemosensitivity assay (ATP-TCA) has shown strong correlation with outcome in cutaneous melanoma, but requires fresh tissue and dedicated laboratory facilities.

Aim To examine whether the results of the ATP-TCA correlate with the expression of genes known to be involved in resistance to chemotherapy, based on the hypothesis that the molecular basis of chemosensitivity lies within known drug resistance mechanisms.

Method The chemosensitivity of 47 cutaneous melanomas was assessed using the ATP-TCA and correlated with quantitative expression of 93 resistance genes measured by quantitative reverse transcriptase PCR (qRT-PCR) in a Taqman Array after extraction of total RNA from formalin-fixed paraffin-embedded tissue.

Results Drugs susceptible to particular resistance mechanisms showed good correlation with genes linked to these mechanisms using signatures of up to 17 genes. Comparison of these signatures for DTIC, treosulfan and cisplatin showed several genes in common. HSP70, at least one human epidermal growth factor receptor, genes involved in apoptosis (IAP2, PTEN) and DNA repair (ERCC1, XPA, XRCC1, XRCC6) were present for these agents, as well as genes involved in the regulation of proliferation (Ki67, p21, p27). The combinations tested included genes represented in the single agent signatures.

Conclusions These data suggest that melanoma chemosensitivity is influenced by known resistance mechanisms, including susceptibility to apoptosis. Use of a candidate gene approach may increase understanding of the mechanisms underlying chemosensitivity to drugs active against melanoma and provide signatures with predictive value.

  • Melanoma
  • ATP
  • chemosensitivity
  • gene expression
  • RT-PCR
  • chemotherapy
  • PCR

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Footnotes

  • Funding The tissue collection was funded in part by the Wessex Cancer Trust, Bellis House, 11 Westwood Road, Southampton SO17 1DL. The ATP-TCA analyses were funded by CanTech Ltd, c/o Translational Oncology Research Centre, Level F - Pathology Centre, Queen Alexandra Hospital, Portsmouth PO6 3LY; the molecular analysis was funded by Applied Biosystems, Foster City, California, USA, and the analysis performed by staff within TORC funded by CanTech Ltd. KP is funded by the Skin Cancer Research Fund, Department of Plastic Surgery, Frenchay Hospital, Bristol BS16 1LE.

  • Competing interests IAC is a Director of CanTech Ltd, which part funded this project.

  • Ethics approval This study was conducted with the approval of the North West Research Ethics Committee, NHS, North West Room, 155 Gateway House, Piccadilly, South Manchester M60 7LP, UK.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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