Article Text

PDF
Angiogenic ability of metastatic squamous carcinoma in the cervical lymph nodes from unknown primary tumours
  1. Beamon Agarwal1,
  2. Pragnya Das2,
  3. Kikkeri N Naresh3,
  4. Anita M Borges4
  1. 1Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, USA
  2. 2Department of Pathology, Children's Hospital of Philadelphia, Philadelphia, USA
  3. 3Department of Hematology, Hammersmith Hospital, London, UK
  4. 4Department of Pathology, Asian Institute of Oncology, Mumbai, India
  1. Correspondence to Dr Beamon Agarwal, University of Pennsylvania School of Medicine, Institute for Diabetes, Obesity, and Metabolism, Department of Physiology, 415 Curie Blvd, CRB 730, Philadelphia, PA 19104, USA; beamon{at}mail.med.upenn.edu

Abstract

Objectives To study angiogenesis in metastasis of unknown primary (MUP) to support the authors' hypothesis that MUPs are of a low angiogenic phenotype and are able to undergo metastasis in spite of an unknown primary.

Patients and methods A retrospective analysis was performed on paraffin blocks obtained from 50 cases of MUP and 52 cases of metastasis of known primary (MKP) from 1 January 2000 to December 2003. A prospective analysis was performed on fresh tissues from 22 cases of MUP and 26 cases of MKP. Immunohistochemical staining for VEGF was performed on the paraffin blocks. The fresh frozen tissue was analysed by RT-PCR and Western blotting for VEGF isoforms (VEGF121, VEGF165, VEGF189) and VEGF protein, respectively.

Results Immunohistochemistry showed that MUPs had a higher percentage of lower scores of VEGF expression than MKPs. MKPs had increased scores of VEGF expression. RT-PCR analysis showed that MKPs had increased expression of VEGF121 and VEGF165 isoforms as compared with MUPs. MKPs showed a higher VEGF protein expression than MUPs.

Conclusion The study shows that metastases of squamous carcinoma from unknown primary have decreased VEGF expression at the immunohistochemical and protein level. They also display decreased expression of the VEGF121 and VEGF165 isoforms. Hence, these tumours are of a low angiogenic phenotype. They are able to develop a metastatic phenotype and grow at the metastatic site, since angiogenesis is redundant for lymph-node metastasis.

  • VEGF
  • unknown primary
  • squamous carcinoma
  • angiogenesis
  • tumour angiogenesis
  • tumour biology
  • neoplasms
  • molecular pathology
  • oncology

Statistics from Altmetric.com

Footnotes

  • Competing interests None.

  • Patient consent Obtained.

  • Ethics approval Ethics approval was provided by the ethical committee of Institutional Review Board.

  • Provenance and peer review Not commissioned; externally peer reviewed.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.