Two cases of opportunistic parasite infections in patients receiving alemtuzumab
- Guillaume Desoubeaux1,
- Charline Caumont1,
- Christophe Passot2,
- Caroline Dartigeas3,
- Éric Bailly1,
- Jacques Chandenier1,
- Thanh Haï Duong1
- 1Unit of Parasitology - Mycology - Tropical Medicine, CHRU Bretonneau, Tours, France
- 2Immunology Laboratory, CHRU Bretonneau, Tours, France
- 3Service of Haematology and Cellular Therapy, CHRU Bretonneau, Tours, France
- Correspondence to Dr Guillaume Desoubeaux, Unit of Parasitology - Mycology - Tropical Medicine, University Hospital Bretonneau, 2 Blvd Tonnellé, Bâtiment B2A, 37044 Tours Cedex 09, France;
Contributors Study concept and design: GD, JC. Acquisition of data: GD, CC, CD, JC. Analysis and interpretation of data: GD, CC, ?Duong, JC. Drafting of the manuscript: GD, ?Duong, JC. Critical revision of the manuscript for important intellectual content: GD, CC, CP, EB, ?Duong, JC. Administrative, technical or material support: GD, JC. Study supervision: GD, JC. All authors had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.
- Accepted 20 September 2011
- Published Online First 3 November 2011
Two cases are reported of rare digestive opportunistic parasites in patients being treated with alemtuzumab for lymphoid haematological malignancies. In both patients, classical biological examinations were insufficient to reach the diagnosis. Only specific parasitological techniques enabled diagnoses of cryptosporidiosis and microsporidiosis, respectively. In both cases, cellular immune reconstitution was sufficient to eradicate these opportunistic infections. In this context, parasitological diagnosis is often underestimated by medical practitioners, so immunologists and oncohaematologists need to be aware of this kind of opportunistic pathogen.
- lymphoid haematological malignancies
- digestive opportunistic parasitoses
- monoclonal antibody
Numerous digestive complications—infectious or non-infectious—may arise during treatment with alemtuzumab (Campath; Genzyme, USA).1–3 Among these, two rare parasites which are well-known in departments of infectious diseases, particularly during the HIV/AIDS outbreak, may be suspected. Case reports of two patients treated with alemtuzumab in whom opportunistic parasitic infections developed are described.
A 75-year-old woman was monitored for stage C chronic lymphocytic leukaemia (CLL) initially diagnosed in 2005. She was started on a fourth course of treatment with alemtuzumab at the end of March 2011 and pancytopenia with lymphocyte depletion (measured values 0–0.610×109/l) was noticed on regular complete blood counts. In May 2011 she presented with a 2-week history of diarrhoea with at least three watery stools per day. She had not undertaken any foreign travel. All the routine tests were negative (bacterial culture, virus research by immunological or molecular methods, standard stool analysis for parasites and anatomopathological examinations of bowel biopsies). As the stools were very liquid, the parasitology laboratory decided to perform further investigations. The temporary wet mount and two concentration techniques (Bailenger's sedimentation and Kato-Katz's techniques) were negative, but Ziehl–Neelsen staining revealed the presence of Cryptosporidium oocysts on smears of methanol-fixed faeces (figure 1) and C meleagridis was identified upon 18S rRNA gene sequencing. Treatment of symptoms and an increase in lymphocyte count after stopping alemtuzumab treatment resulted in a favourable outcome within a few weeks.
A 77-year-old man presented with uncontrolled CLL after five sessions of chemotherapy. Alemtuzumab treatment was started in January 2011. Complete blood counts showed lymphocyte counts of 0.3–0.8×109/l from January to June 2011. Since starting treatment with alemtuzumab, recurrent aqueous diarrhoea had occurred. The patient had not undertaken any foreign travel. Routine biological tests were negative. The only test with a positive result was Enterocytozoon bieneusi real-time PCR with internal positive control performed on a stool sample (LightCycler 480, Roche Diagnostics, France) (figure 2). The microsporidial gene fragment targeted was the SSU rDNA. Designed Eb1 (5′-CGACAGCCTGTGTGTGAGAATAC) and Eb5 (5′-CAACGAATGACTTGACCCTGGTAA) were used as primers and EbS2 (6FAM TGCTTAATTTAACTCAACGCGGGAAAA TAMRA) was chosen as the fluorescent TaqMan probe. Parasitological confirmation was made with Weber's Trichrome blue stain (figure 3) and van Gool's Uvitex 2B fluorescent brightener (figure 4) which enabled detection of microsporidia. As the lymphocyte count reached the value of 1×109/l, the diarrhoea gradually decreased without specific treatment. Neither a whole microsporidial microorganism nor DNA was detected in later stool samples after total cellular immune reconstitution obtained within 3 months.
Microorganisms of the genuses Enterocytozoon and Cryptosporidium are ubiquitous unicellular eukaryotes whose epidemiology is often linked to the quality of the water source.4 5 The former belongs to the Microsporidia group and the latter are coccidia of the Apicomplexa phylum. Until the middle of the 1990s, microsporidiosis and cryptosporidiosis had been described during episodes of diarrhoea primarily in HIV-positive persons with AIDS. Since the advent of the highly active antiretroviral therapies in 1996, such infections have become rare in treated persons. At present, few cases are diagnosed in non-HIV subjects, particularly those undergoing solid organ transplantation/bone marrow graft or those with leukaemia.6–9 Although the number of cases remains small, these opportunistic parasites can be highly pathogenic, especially when the number of CD4 T lymphocytes is <0.2×109/l (note also that immunocompetent subjects may be sporadically affected by cryptosporidiosis although the pathogenesis is appreciably different from in immunocompromised patients). As soon as it is suspected, the diagnosis of cryptosporidiosis is easy and almost all laboratories should be able to perform correct staining for Cryptosporidium. In contrast, very few laboratories can provide a reliable microscopic diagnosis of microsporidiosis.
For the last 20 years the use of monoclonal antibodies has provided a significant improvement in the medical management of cancer and autoimmune diseases. Alemtuzumab is a humanised monoclonal antibody derived from rat. This recombinant IgG1K targets CD52, a 21–28 kDa surface glycoprotein seen particularly on mature lymphocytic cells. It is thought to work via the activation of antibody-dependent cell-mediated cytotoxicity10 and/or complement cytotoxicity.11 This drug is indicated in the treatment of relapsing or complicated CLL12 and in some kinds of non-Hodgkin's lymphoma. Alemtuzumab is also sometimes used in some conditioning regimens for bone marrow graft13 or solid organ transplantation.14 It has also recently been investigated in a phase II clinical trial in the treatment of relapsing-remitting multiple sclerosis.15 One of the major known potential adverse events of alemtuzumab is the increased risk for onset of opportunistic infections due to intense lymphocyte depletion associated with profound cellular immune dysfunction.16–18 No correlation exists between the cumulative dose of alemtuzumab and the length or severity of immunodepletion. Many reactivations with herpes or hepatitis viruses have been reported, especially shortly after the T cell nadir. For instance, cytomegalovirus reactivation is described in 10–25% of patients receiving alemtuzumab after about 4–6 weeks of treatment.19 20 A few bacterial,2 fungal21 or even parasitic infections22 have also been reported.1 3 23 This potential risk therefore requires investigation of the viral serological status and bacterial colonisation index. In this context, prophylaxis against various infectious agents should be given until immune reconstitution.18 24–26
In contrast, infrequent digestive complications due to unicellular pathogens such as microsporidia or cryptosporidia are less well-known and are often misdiagnosed during treatment with alemtuzumab. To our knowledge, very few cases of microsporidiosis have been reported in patients treated with alemtuzumab.27 No report has been found of cryptosporidiosis caused by this monoclonal antibody.23 In this context, the risk of such rare opportunistic parasites is not well known so it is not sought. However, it appears to be theoretically realistic, even if there has been no travel in tropical countries. Usually, when bowel symptoms are observed, digestive graft-versus-host disease, drug toxicity or bacterial/viral infections are suspected. As in our case reports, a diagnosis of digestive parasitic infections usually involves excluding other illnesses. According to the Crypto-ANOFEL French network recommendations, specific parasitological techniques should be performed in cases of liquid diarrhoea occurring during immunodepletion. For these two patients, the laboratory used Ziehl–Neelsen staining for cryptosporidia and Weber staining for microsporidia detection followed by confirmation with fluorescence.28 In our laboratory, molecular methods, which are more sensitive, are recommended for easier screening of small pathogens such as microsporidia E bieneusi and are therefore used automatically first on every liquid stool. This method is now standardised, validated and can also be performed with many real-time machines (Roche (France), Applied Biosystems).
These cases strengthen the need to consider such environmental eukaryotes as potential pathogens in immunocompromised subjects, including unusual patients from oncohaematology treated with monoclonal antibodies such as alemtuzumab. A knowledge of the broad panel of potential infectious agents enables a better approach for clinicians.23 A delay in the time to diagnosis can impact on the success of the medical care. A few antiparasitic treatments have been used with interesting but controversial successes (eg, nitazoxanide for cryptosporidia, fumagillin for Enterocytozoon spp. microsporidia).29 30
Our findings suggest that patients treated with alemtuzumab must be considered a new group of patients with increased susceptibility to this kind of microorganism, even if they have not travelled abroad. They should be monitored closely, with an emphasis on researching opportunistic infections—including parasitological infections—especially around the fourth week of treatment when the lymphocyte number is lowest.
During therapies including monoclonal antibodies, opportunistic parasits should be suspected as digestive symptoms have began, especially when they last a few weeks, in spite of antibiotics.
The authors thank all the personnel in the Parasitology and Haematology laboratories for their collaboration.
Competing interests None.
Patient consent Obtained.
Provenance and peer review Not commissioned; externally peer reviewed.