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Evaluation of three selective chromogenic media, CHROMagar ESBL, CHROMagar CTX-M and CHROMagar KPC, for the detection of Klebsiella pneumoniae producing OXA-48 carbapenemase
  1. Michael Hornsey1,
  2. Lynette Phee1,2,
  3. Neil Woodford1,3,
  4. Jane Turton3,
  5. Daniele Meunier3,
  6. Claire Thomas4,
  7. David W Wareham1,2
  1. 1Antimicrobial Research Group, Centre for Immunology and Infectious Disease, Blizard Institute, Barts & The London School of Medicine and Dentistry, Queen Mary University London, London, UK
  2. 2Division of Infection, Barts Health NHS Trust, London, UK
  3. 3Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Health Protection Agency, Microbiology Services, Colindale, London, UK
  4. 4Hammersmith Hospital NHS Trust, London, UK
  1. Correspondence to Dr David W Wareham, Antimicrobial Research Group, Centre for Immunology and Infectious Disease, Blizard Institute, 4 Newark Street, Whitechapel, London E1 2AT, UK; d.w.wareham{at}qmul.ac.uk

Abstract

Three selective chromogenic culture media (CHROMagars ESBL, CTX-M and KPC) were evaluated for their ability to support the growth of nine Klebsiella pneumoniae isolates producing OXA-48 carbapenemase in combination with other β-lactamases. CHROMagar ESBL and CHROMagar KPC were the most sensitive media, supporting growth of all isolates with a detection limit as low as < 100 CFU/ml. Five isolates failed to grow on CHROMagar CTX-M, and five were recovered on CHROMagar KPC only at counts > 106 CFU/ml. Both CHROMagar ESBL and CHROMagar KPC may be useful for enhanced isolation of K pneumoniae producing OXA-48-like carbapenemases.

  • Antimicrobial Resistance
  • Bacteriology
  • Methodology

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