Article Text

PDF
Correspondence
Gene amplified in oesophageal cancer 1 (GAEC1) amplification in colorectal cancers and its impact on patient's survival
  1. Vinod Gopalan1,
  2. Koichi Yasuda1,
  3. Suja Pillai1,
  4. Thomas Tiang1,
  5. Melissa Leung1,
  6. Cu-Tai Lu1,
  7. Johnny Chuek-on Tang2,
  8. Robert A Smith1,
  9. Alfred King-Yin Lam1
  1. 1Cancer Molecular Pathology, School of Medicine and Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia
  2. 2Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, Hong Kong SAR
  1. Correspondence to Professor Alfred Lam, Cancer Molecular Pathology, School of Medicine and Griffith Health Institute, Griffith University, Gold Coast Campus, Gold Coast QLD 4222, Australia; a.lam{at}griffith.edu.au

Statistics from Altmetric.com

GAEC1 (gene amplified in oesophageal cancer 1) is located at 7q22.1, first identified in oesophageal cancer.1 Initial work indicated that GAEC1 can act as an oncogene.2 Our pilot study found ∼80% of colorectal cancers showing amplification of GAEC1.3 In this research, we will study GAEC1 copy number in colon cancer cell lines and colorectal tissues, and its prognostic significance.

Two human colon cancer cell lines (SW480 and SW48) and one normal colonic epithelial cell line (FHC) were obtained from American Type Culture Collection. Culturing conditions for these cell lines were as published previously.4 Tissues were collected from 283 patients (213 Australian; 70 Japanese) diagnosed with colorectal cancers. Ninety surgically removed non-cancer colorectal tissues (diverticular diseases, hyperplastic polyps and volvulus) were used as controls. H&E stained sections from each cancer were checked to select a block with sufficient cancer tissue and representative morphological features for each patient for DNA extraction.

Cancers were staged according to the Union for International Cancer Control (UICC) for TNM (tumour, node and metastasis) classification.5 Only primary adenocarcinomas were included. Actuarial survival rates of patients were calculated from the date of surgical resection of the colorectal cancers to the date of death or last follow-up. DNA extraction and PCR reactions for GAEC1 copy number were performed as in our previous studies.3 ,4 A ΔCt of <−1 was considered as gain of GAEC1 copies, Ct value >1 was considered as reduced GAEC1 copies and a Ct between these ranges was defined as normal/no change in GAEC1 …

View Full Text

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.