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Microvascular proliferation in luminal A and basal-like breast cancer subtypes
  1. Maria Ryssdal Kraby1,
  2. Kristi Krüger2,
  3. Signe Opdahl3,4,
  4. Lars J Vatten4,
  5. Lars A Akslen2,5,
  6. Anna M Bofin1
  1. 1Department of Laboratory Medicine, Children's and Women's Health, Norwegian University of Science and Technology, Trondheim, Norway
  2. 2Department of Clinical Medicine, Centre for Cancer Biomarkers, University of Bergen, Bergen, Norway
  3. 3Central Norway Regional Health Authority, Trondheim, Norway
  4. 4Department of Public Health and General Practice, Norwegian University of Science and Technology, Trondheim, Norway
  5. 5Department of Pathology, Haukeland University Hospital, Bergen, Norway
  1. Correspondence to Maria Ryssdal Kraby, Department of Laboratory Medicine, Children's and Women's Health, Norwegian University of Science and Technology, Erling Skjalgssons gate, Trondheim 7030, Norway; kraby{at}stud.ntnu.no

Abstract

Aims The aims of this study were to examine microvessel density (MVD), proliferating MVD (pMVD) and Vascular Proliferation Index (VPI) in basal-like phenotype (BP) and luminal A subtypes of breast cancer and to study their prognostic value.

Methods Dual-colour immunohistochemistry for von Willebrand factor and Ki67 was done on sections from 62 luminal A and 62 BP tumours matched for grade and selected from 909 breast cancers previously reclassified into molecular subtypes. Associations between MVD, pMVD and VPI, molecular subtypes and breast cancer prognosis were estimated using linear regression and survival analyses.

Results Both pMVD (difference 1.9 microvessels/mm2 (p=0.002)) and VPI (difference 1.7 percentage points (p=0.014)) were higher in BP tumours compared with luminal A. No clear difference between subtypes was found for MVD. However, only MVD was associated with prognosis. HR for breast cancer death for all cases was 1.10 (95% CI 1.02 to 1.18) per 10 vessels increase. Among luminal A tumours, HR was 1.22 per 10 vessels increase (p<0.001) and in BP it was 1.04 (p=0.37).

Conclusions High MVD was associated with poor prognosis in luminal A, but not in BP cancers. Vascular proliferation was higher in BP, indicating a more active angiogenesis than in luminal A tumours. The luminal A subgroup comprised mostly histopathological grade 3 cancers in this selected series, and further studies are needed to clarify whether MVD provides additional prognostic information for luminal A tumours irrespective of grade. This may contribute to stratification of this large group of patients and may aid in identifying tumours with a particularly good prognosis.

  • ANGIOGENESIS
  • BREAST CANCER
  • IMMUNOHISTOCHEMISTRY
  • BREAST PATHOLOGY
  • CANCER RESEARCH

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