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miR-19a and SOCS-1 expression in the differential diagnosis of laryngeal (glottic) verrucous squamous cell carcinoma
  1. Gino Marioni1,
  2. Marco Agostini2,3,4,
  3. Rocco Cappellesso5,
  4. Chiara Bedin2,3,
  5. Giancarlo Ottaviano1,
  6. Rosario Marchese-Ragona1,
  7. Andrea Lovato1,
  8. Tommaso Cacco1,
  9. Luciano Giacomelli5,
  10. Donato Nitti2,
  11. Stella Blandamura5,
  12. Edoardo Stellini6,
  13. Cosimo de Filippis7
  1. 1Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy
  2. 2Department of Surgical, Oncological and Gastroenterological Sciences, Padova University, Padova, Italy
  3. 3Istituto di Ricerca Pediatrica-Città della Speranza, Padova, Italy
  4. 4Nanomedicine Department, The Methodist Hospital Research Institute, Houston, Texas, USA
  5. 5Department of Medicine DIMED, University of Padova, Padova, Italy
  6. 6Department of Neurosciences DNS, Odontostomatology Institute, Padova University, Padova, Italy
  7. 7Department of Neurosciences DNS, Audiology Unit, Treviso Hospital, Padova University, Treviso, Italy
  1. Correspondence to Professor Gino Marioni, Department of Neurosciences DNS, Otolaryngology Section, University of Padova, Via Giustiniani 2, Padova 35128, Italy; gino.marioni{at}unipd.it

Abstract

Background Laryngeal verrucous squamous cell carcinoma (VSCC) is a highly differentiated squamous cell carcinoma (SCC), the diagnosis of which can meet with many pitfalls: benign hyperplastic lesions and conventional SCC are the most important differential diagnoses. The microRNA miR-19a is overexpressed in many solid tumours and regulates the suppressor of cytokine signalling-1 (SOCS-1) expression.

Aims The main endpoints were to assess miR-19a and SOCS-1 expression in glottic VSCC, and the former's potential role in differentiating between glottic VSCC, conventional SCC and hyperplastic lesions.

Methods The expression of MiR-19a (by reverse transcription and quantitative real-time PCR) and SOCS-1 (by immunohistochemistry, rabbit polyclonal anti-SOCS-1 antibody) was assessed in 11 consecutive cases of glottic VSCC, 20 of papillary hyperplasia and 42 cases of conventional SCC.

Results Mean miR-19a expression was significantly higher (p=0.000) in malignant glottic lesions (conventional SCC/VSCC) than in benign conditions. Significant differences in mean miR-19a expression also emerged between conventional SCC and papillary hyperplasia (p=0.000), and between conventional SCC and VSCC (p=0.03). miR-19a expression was not statistically associated with SOCS-1 immunoreactivity or immunostaining intensity in VSCC, conventional SCC or papillary hyperplasia.

Conclusions Our preliminary outcomes suggest the utility of miR-19a in the challenging differential diagnosis of laryngeal VSCC. Although miR-19a has been found to regulate SOCS-1 expression, this evidence was not confirmed by this investigation.

  • LARYNGEAL CANCER
  • TUMOUR MARKERS
  • CANCER

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