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EGFR mutation detection on routine cytological smears of non-small cell lung cancer by digital PCR: a validation study
  1. Umberto Malapelle1,
  2. Caterina de Luca1,
  3. Elena Vigliar1,
  4. Francesca Ambrosio2,
  5. Danilo Rocco3,
  6. Pasquale Pisapia1,
  7. Claudio Bellevicine1,
  8. Giancarlo Troncone1
  1. 1Department of Public Health, University of Naples Federico II, Naples, Italy
  2. 2Azienda Ospedaliera di Rilievo Nazionale, Antonio Cardarelli, Naples, Italy
  3. 3Azienda Ospedaliera di Rilievo Nazionale, Ospedale dei Colli, Naples, Italy
  1. Correspondence to Professor Giancarlo Troncone, Department of Public Health University of Naples Federico II, via Sergio Pansini 5, Naples I-80131, Italy; giancarlo.troncone{at}unina.it

Abstract

Highly sensitive genotyping techniques are useful to detect epidermal growth factor receptor (EGFR) mutations on lung cancer cytological samples, when these specimens feature only few neoplastic cells. This study aimed to validate digital PCR (dPCR) methodology on cytological material. In plasmid model system, dPCR allowed for the detection of a minimal percentage (1%) of EGFR mutant alleles. Cytological samples (n=30), with neoplastic cell percentage ranging from 10% to 80% and yielding a quantity of extracted DNA ranging from 1.75 to 60 ng/µL were selected. Results previously generated by fragment length and TaqMan assays (n=8 exon 19 deletions, n=2 L858R mutations and n=20 wild-type DNA) were compared with those obtained by dPCR. Data were highly concordant (96.6%). However, dPCR detected an additional L858R mutation that had been missed by TaqMan assay on a paucicellular smear. This mutation was confirmed by cloning PCR products and sequencing. Thus, dPCR can reliably be used to increase EGFR mutation detection rate on scarcely cellular lung cancer smears.

  • LUNG CANCER
  • EGFR
  • CYTOPATHOLOGY
  • PCR

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