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Correlation of ERG immunohistochemistry with molecular detection of TMPRSS2-ERG gene fusion
  1. Ji-Youn Sung1,
  2. Hwang Gyun Jeon2,
  3. Byong Chang Jeong2,
  4. Seong Il Seo2,
  5. Seong Soo Jeon2,
  6. Hyun Moo Lee2,
  7. Han Yong Choi2,
  8. So Young Kang3,
  9. Yoon-La Choi3,
  10. Ghee Young Kwon3
  1. 1Department of Pathology, Kyung Hee University Hospital, Kyung Hee University School of Medicine, Seoul, Korea
  2. 2Department of Urology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
  3. 3Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
  1. Correspondence to Dr Ghee Young Kwon, Department of Pathology, Samsung Medical Center, School of Medicine, Sungkyunkwan University, 50 Irwon-dong, Gangnam-gu, Seoul 135–710, Korea; geeo{at}skku.edu

Abstract

Aims TMPRSS2/E26 transformation-specific (ETS) family gene fusion in prostate carcinoma (PCa) can be detected by several methods including immunohistochemistry (IHC) for ETS-related gene (ERG), the diagnostic utility of which has not been clearly defined.

Methods We explored TMPRSS2-ERG gene rearrangement status in 132 patients with PCa with four detection methods including fluorescence in situ hybridisation for TMPRSS2-ERG fusion, real-time reverse transcription PCR (RT-qPCR) for ERG and TMPRSS-ERG fusion transcript mRNA and IHC for ERG.

Results Concordant results were found in 126 cases for the four detection methods and the remaining six cases showed discrepancy in one method: two cases in IHC, three cases in RT-qPCR for ERG and one case in RT-qPCR for fusion transcript. In discordant cases, the majority results were determined as final fusion status. Analysis of discrepancy cases for ERG IHC showed that weak immunoreactivity for ERG should be regarded as equivocal and that even strong immunoreactivity can be false positive. The overall incidence of TMPRSS-ERG gene fusion was 24%.

Conclusions ERG IHC is a useful surrogate test for the detection of TMPRSS2-ERG gene fusion, but it needs to be interpreted with caution and definite judgement should not be based on IHC alone. A relatively low incidence of TMPRSS2-ERG gene fusion was demonstrated in this Korean cohort.

  • PROSTATE
  • IMMUNOHISTOCHEMISTRY
  • MOLECULAR PATHOLOGY

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