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Comparative evaluation of six chromogenic media for presumptive yeast identification
  1. Alessandra Vecchione1,
  2. Walter Florio1,
  3. Francesco Celandroni1,
  4. Simona Barnini2,
  5. Antonella Lupetti1,
  6. Emilia Ghelardi1
  1. 1 Dipartimento di Ricerca Traslazionale e delle Nuove Tecnologie in Medicina e Chirurgia, Università di Pisa, Pisa, Italy
  2. 2 Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy
  1. Correspondence to Dr Antonella Lupetti, Dipartimento di Ricerca Traslazionale e delle Nuove Tecnologie in Medicina e Chirurgia, Università di Pisa, Via San Zeno 37, 56127, Pisa, Italy; antonella.lupetti{at}med.unipi.it

Abstract

Aims The present study was undertaken to evaluate the discrimination ability of six chromogenic media in presumptive yeast identification.

Methods We analysed 108 clinical isolates and reference strains belonging to eight different species: Candida albicans,Candida dubliniensis, Candida tropicalis, Candida krusei, Candida glabrata, Candida parapsilosis,Candida lusitaniae and Trichosporon mucoides.

Results C. albicans, C. tropicalis and C. krusei could be distinguished from one another in all the tested chromogenic media, as predicted by the manufacturers. In addition, C. albicans could be distinguished from C. dubliniensis on BBL CHROMagar Candida, Kima CHROMagar Candida and Brilliance Candida, and C. parapsilosis could be identified on CHROMATIC Candida agar, CHROMOGENIC Candida agar, and Brilliance Candida agar.

Conclusions Brilliance Candida provided the widest discrimination ability, being able to discriminate five out of the seven Candida species tested. Interestingly, C. tropicalis and C. krusei could be already distinguished from each other after 24 hours of incubation.

  • chromogenic media
  • yeast identification
  • CandiSelect 4
  • CHROMagar Candida
  • CHROMATIC Candida
  • CHROMOGENIC Candida agar

https://doi.org/10.1136/jclinpath-2017-204396

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    Footnotes

    • Handling editor Tahir S Pillay

    • Contributors Conceptualisation: AV, SB and EG. Experiments execution and analysis of results: AV, WF and FC. Manuscript draft: WF, AL and EG.

    • Funding This study was supported by a research grant from the Italian “Ministero dell’Istruzione, dell’Università e della Ricerca”, contract n° 2012WJSX8K_005.

    • Competing interests None declared.

    • Ethics approval Ethical Committee ’Comitato Etico di Area Vasta Nord-Ovest', University of Pisa.

    • Provenance and peer review Not commissioned; externally peer reviewed.