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Evaluation of a fast and fully automated platform to diagnose EGFR and KRAS mutations in formalin-fixed and paraffin-embedded non-small cell lung cancer samples in less than one day
  1. Laetitia Lambros1,
  2. Charline Caumont2,
  3. Briac Guibourg1,
  4. Fanny Barel1,
  5. Isabelle Quintin-Roué1,
  6. Pascale Marcorelles1,3,
  7. Jean-Philippe Merlio2,
  8. Arnaud Uguen1,3,4
  1. 1Department of Pathology, CHRU Brest, Brest, France
  2. 2Department of Tumors Biology, CHU Bordeaux Haut-Lévêque, Pessac, France
  3. 3Inserm, Brest, France
  4. 4European University of Brittany, France
  1. Correspondence to Dr Arnaud Uguen, Department of Pathology, University Hospital Morvan, 5, Avenue Foch 29609 Brest, France; arnaud.uguen{at}chu-brest.fr

Abstract

Aims Searching for EGFR and KRAS mutations within non-small cell lung carcinoma (NSCLC) samples remains time-consuming and can delay treatment choices in patients with acute deterioration. We evaluated the performances of the fully automated Idylla platform to quickly detect these mutations in NSCLC samples.

Methods We used the Idylla EGFR Mutation Assay and the Idylla KRAS Mutation Test to analyse 18 formalin-fixed paraffin-embedded NSCLC tumour samples with known EGFR and KRAS mutation status according to next-generation sequencing (NGS) and droplet digital PCR (ddPCR) for EGFRT790M mutations.

Results Idylla assays identified KRAS and EGFR activating mutations in 4 and 10 NSCLC samples, respectively. EGFRT790M resistance mutations were identified in only 1 sample using Idylla but in 4 and 14 samples using NGS and ddPCR, respectively. No false-positive result was noted with Idylla assays. Mutation written report was obtained after 130 min (KRAS assays) to 140 min (EGFR assays).

Conclusions The Idylla platform is an interesting ancillary first-line fast and fully automated tool to detect EGFR and KRAS mutations in NSCLC samples allowing rapid treatment choices in patients with acute deterioration.

  • LUNG CANCER
  • EGFR
  • PCR
  • MOLECULAR PATHOLOGY

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Footnotes

  • Handling editor Runjan Chetty

  • Contributors Conceptualisation: LL and AU; sample preparation and Idylla assays: LL, FB, BG, IQ-R and AU; ddPCR assays: CC and J-PM; manuscript draft: LL, CC, PM, J-PM and AU.

  • Competing interests None declared.

  • Ethics approval CHRU Brest, CPP no. DC-2008-214.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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