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Tissue factor expression by a human kidney proximal tubular cell line in vitro: A model relevant to urinary tissue factor secretion in disease?
  1. Bashir A Lwaleed (bashir{at}soton.ac.uk)
  1. Department of Urology, Southampton University Hospitals NHS Trust, United Kingdom
    1. Steven Vayro (vayro{at}port.ac.uk)
    1. School of Pharmacy and Biomedical Sciences University of Portsmouth, United Kingdom
      1. Lorraine C. Racusen (rcusen{at}jhmi.edu)
      1. Johns Hopkins Medical School, Baltimore, United States
        1. Alan J Cooper (alancooper{at}port.ac.uk)
        1. School of Pharmacy and Biomedical Sciences University of Portsmouth, United Kingdom

          Abstract

          Aim: In order to establish a model for investigating the role of inflammatory mediators in the increased urinary tissue factor (uTF) seen in inflammatory and neoplastic disease, we have studied baseline and stimulated TF production from a normal, albeit immortalised, human kidney proximal tubular cell line (HKC-5).

          Materials and Methods: Tissue factor (TF) procoagulant activity, expression and secretion in HKC-5 cells were investigated using TF activity and antigen assays, fluorescence confocal microscopy and immunocytochemistry. TF expression in the HKC-5 cells was also studied using reverse transcription-polymerase chain reaction (RT-PCR) and its synthesis was suppressed using antisense oligodeoxynucleotide (ODN), directed against human TF mRNA. Cells were stimulated, after serum deprivation, with bacterial lipopolysaccharide (LPS), an agonist known to enhance TF expression in monocytes. They were also subject to serum starvation.

          Results: Analysis by RT-PCR demonstrated TF production by stimulated and actively metabolising HKC-5 cells. Antisense ODN treatment resulted in approximately 50% suppression of TF synthesis compared to a mismatch ODN. The amount of TF produced by the HKC-5 cells was time dependent and coincides with a decrease in the intracellular TF levels. LPS up regulated TF production in HKC-5 cells. Reducing FCS-concentrations in the culture medium decreased TF production and secretion.

          Conclusion: Stimulated TF synthesis and secretion in vitro by HKC-5 cells is consistent with the hypothesis that uTF is produced by tubular cells influenced by mediators of disease states and provides a model for further mechanistic investigations.

          • Antisense oligodeoxynucleotides
          • Human kidney, Proximal tubules, HKC-5 cell lines
          • Immunostaining
          • RT-PCR
          • Tissue factor

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