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Complex patterns of chromosome 9 alterations including the p16INK4A locus in Wilms tumours
  1. Rachael Natrajan
  1. Institute of Cancer Research, United Kingdom
    1. William Warren
    1. Institute of Cancer Research, United Kingdom
      1. Boo Messahel
      1. Institute of Cancer Research, United Kingdom
        1. Jorge Reis-Filho
        1. Breakthrough Breast Cancer, United Kingdom
          1. Marie-Anne Brundler
          1. Birmingham Children’s Hospital, United Kingdom
            1. Jeff Dome
            1. St Jude Children's Research Hospital, United States
              1. Paul Grundy
              1. University of Alberta, Canada
                1. Gordan Vujanic
                1. University Hospital of Wales, United Kingdom
                  1. Kathy Pritchard-Jones
                  1. Institute of Cancer Research, United Kingdom
                    1. Chris Jones (chris.jones{at}
                    1. Institute of Cancer Research, United Kingdom


                      Aims: Previous data implicating genetic and epigenetic events on chromosome 9, including the CDKN2A/2B locus, as molecular predictors of Wilms tumour relapse, have been conflicting. We wished to clarify this using genome-wide and focussed molecular genetic analysis.

                      Methods: Microarray-based comparative genomic hybridisation (aCGH) using genome-wide coverage was applied to 76 favourable histology Wilms tumours. Additional investigations of the 9p21 locus was carried out using loss of heterozygosity and FISH, as well as immunohistochemistry for CDKN2A/p16INK4a on a paediatric renal tumour tissue microarray.

                      Results: Approximately half of the tumours were found to show chromosome 9 copy number changes. Those cases which harbour alterations comprise at least four distinct patterns – gain of the entire chromosome, loss of 9p, gain of 9q34, or a more complex combination of gains/losses. None of these tumour groups showed any statistically significant correlation with clinicopathological variables. Deletion mapping of 9p by LOH revealed several regions of overlap, including the CDKN2A/2B locus in 4/34 (11.8%) of tumours, which was confirmed to represent hemizygous deletions by FISH. CDKN2A/p16INK4a protein expression was predominantly negative in Wilms tumours as assessed by immunohistochemistry on a tissue array, reflecting the expression pattern in normal kidney. However, 38/236 (16.1%) favourable histology Wilms tumours, 4/9 (44.4%) anaplastic Wilms tumours, 5/7 (71.4%) rhabdoid tumours of the kidney, and 4/10 (40%) clear cell sarcomas of the kidney showed nuclear CDKN2A/p16INK4a immunoreactivity.

                      Conclusions: These data reveal the complex nature of genetic alterations on chromosome 9 in Wilms tumours, but do not provide evidence for their involvement in or association with treatment failure.

                      • 9q34
                      • CDKN2A/p16INK4a
                      • LOH
                      • Wilms tumour
                      • trisomy 9

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