Article Text

other Versions

PDF
Expression of ZNF652, a novel zinc finger protein, in vulvar carcinomas and its relation to prognosis.
  1. Ruth Holm (ruth.holm{at}radiumhospitalet.no)
  1. Rikshospitalet-Radiumhospitalet Medical Center, Norway
    1. Synne Knopp (synnes{at}gmail.com)
    1. Rikshospitalet-Radiumhospitalet Medical Center, Norway
      1. Raman Kumar (raman.sharma{at}imvs.sa.gov.au)
      1. University of Adelaide and Hanson Institute, Australia
        1. Jaclyn Lee (jaclyn.lee{at}imvs.sa.gov.au)
        1. University of Adelaide and Hanson Institute, Australia
          1. Jahn M. Nesland (j.m.nesland{at}medisin.uio.no)
          1. Rikshospitalet-Radiumhospitalet Medical Center, Norway
            1. Claes Tropè (c.g.trope{at}klinmed.uio.no)
            1. Rikshospitalet-Radiumhospitalet Medical Center, Norway
              1. David F. Callen (david.callen{at}imvs.sa.gov.au)
              1. University of Adelaide and Hanson Institute, Australia

                Abstract

                Aims: To determine the levels of expression of ZNF652 and its relevance to prognosis in vulvar squamous cell carcinomas.

                Methods: Twenty-two cases of vulvar intraepithelial neoplasia (VIN) and tumors from 217 patients with vulvar squamous cell carcinomas were investigated for expression of ZNF652 using immunostaining methods. The effect of ZNF652 ectopic expression was determined in the vulvar carcinoma cell line SW954 by Western and cell-based assays.

                Results: High levels of ZNF652 nuclear expression were observed in 5 (100%) of VIN I, 6 (75%) of VIN II and 109 (50.2%) of the vulvar carcinomas, whereas low levels were seen in 2 (25%) VIN II, 9 (100%) of VIN III and 108 (49.8%), of the vulvar carcinomas. High levels of ZNF652 expression in the vulvar carcinomas were significantly correlated to high expression of EphA2. However, when correcting for multiple testing this correlation was lost. No association was identified between ZNF652 expression and p16, p21, p27, p53, cyclin A, D1, D3, E, EphrinA-1 and HPV. Variations in levels of ZNF652 were not related to prognosis. Low levels of ZNF652 protein were identified in the vulvar carcinome cell line SW954. Furthermore, SW954 cells ectopically expressing ZNF652 showed reduced cell proliferation and the ability to form colonies on plastic.

                Conclusions: ZNF652 protein expression is reduced in 25% of VIN II, 100% of VIN III and approximately 50% of the cases of vulvar squamous cell carcinoma and may be an early event in the pathogenesis of vulvar squamous cell carcinoma. Variations in the levels of ZNF652 were not related to patient’s prognosis.

                • Immunohistochemistry
                • Vulvar cancer
                • Zinc finger protein

                Statistics from Altmetric.com

                Request permissions

                If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.