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Quantitation of intrahepatic total hepatitis B virus dna in chronic hepatitis B patients and its relationship with liver histology
  1. Aysen Bayram (aysenbayram{at}
  1. Gaziantep University, Turkey
    1. Suna Erkilic
    1. Gaziantep University, Turkey
      1. Ayhan Özkur
      1. Gaziantep University, Turkey
        1. Metin Bayram
        1. Gaziantep University, Turkey
          1. Ibrahim Sari
          1. Gaziantep University, Turkey


            Aims: This study was conducted to evaluate the relationship between total intrahepatic hepatitis B virus (HBV) DNA levels and liver histology in term of the degree of histologic activity index (HAI) which yields necroinflammation (HAI-NI) and fibrosis (HAI-F) of the liver.

            Methods: Prospectively, Tru-cut needle biopsy samples were obtained from liver of 42 chronic hepatitis B patients. Serum and liver HBV DNA levels were determined by quantitative real-time polymerase chain reaction (RT-PCR) method. Demographic data of patients together with hepatitis B serology, alanine aminotransferase (ALT) levels, HAI-NI, and HAI-F scores of the patients were recorded.

            Results: Twenty of the patients were hepatitis B e antigen (HBeAg)-positive, while 22 patients were positive for antibody to HBeAg (anti-HBe). Serum and liver total HBV DNA levels were found to correlate directly with each other in both groups (r=0.669, p=0.001 and r=0.880, p<0.001, respectively) which was more marked in anti-HBe-positive patients. Although serum HBV DNA levels were correlated positively with HAI-NI and HAI-F scores in both HBeAg-positive and HBeAg-negative patients, total liver HBV DNA levels were correlated with HAI-NI and HAI-F scores only in anti-HBe-positive patients.

            Conclusions: We conclude that quantitative measurement of intrahepatic HBV DNA is a valuable marker of the histologic status of the liver in anti-HBe-positive chronic hepatitis B patients, which may give insight for the prognosis and ideal time for the cessation of antiviral treatment.

            • chronic hepatitis
            • hepatitis B virus
            • liver histology
            • real-time PCR

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