Article Text

other Versions

PDF
FOXP3 immunohistochemistry on formalin-fixed paraffin embedded tissue- poor correlation between different antibodies.
  1. Yin Ling Woo (ylw22{at}cam.ac.uk)
  1. University of Cambridge, United Kingdom
    1. Jane C Sterling (jcs12{at}mole.bio.cam.ac.uk)
    1. University of Cambridge, United Kingdom
      1. Robin AF Crawford (robin.crawford{at}addenbrookes.nhs.uk)
      1. Addenbrooke's Hospital, United Kingdom
        1. Sjoerd H van der Burg (s.h.van_der_burg{at}lumc.nl)
        1. Leiden University Medical Centre, Netherlands
          1. Nicholas Coleman (nc109{at}cam.ac.uk)
          1. University of Cambridge, United Kingdom
            1. Margaret A Stanley (mas{at}mole.bio.cam.ac.uk)
            1. University of Cambridge, United Kingdom

              Abstract

              Since its original description, there has been a substantial output of publications related to the FOXP3 gene. FOXP3 protein, a member of the forkhead/winged-helix family of transcriptional regulators is a nuclear product and is not expressed in the cell cytoplasm or surface. Expression of this single transcription factor causes a developmental switch in naïve T-cells to a suppressor cell phenotype, more commonly referred to as regulatory T-cells (Tregs). Tregs are now intensively studied in various autoimmune diseases, infections and different cancers. An increasing choice of commercially available monoclonal antibodies targeting FOXP3 is now available. Here, we report our experience in using two commonly used monoclonal FOXP3 antibodies on formalin- fixed paraffin-embedded sections of different organs including the cervix and vulva. The antibodies targeting different FOXP3 epitopes unexpectedly resulted in significantly different staining patterns. This phenomenon has not been previously reported and is likely to be an important observation.

              Statistics from Altmetric.com

              Request permissions

              If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.