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Histological scoring of fibrosis and activity in HIV-chronic hepatitis B-related liver disease: performance of the METAVIR score assessed on virtual slides.
  1. Dominique Wendum (dominique.wendum{at}sat.ap-hop-paris.fr)
  1. Hôpital Saint-Antoine, France
    1. Karine Lacombe
    1. UPMC University Paris, APHP Hôpital St Antoine and Inserm UMR-S707 Paris, France
      1. Michèle Chevallier
      1. Laboratoire Marcel Mérieux, Lyon, France
        1. Patrice Callard
        1. APHP Hôpital Tenon and UPMC University Paris, France
          1. Fabien Valet
          1. APHP Hôpital St Louis, France
            1. Patrick Miailhes
            1. Hospices civils de Lyon, France
              1. Philippe Bonnard
              1. APHP Hôpital St Louis, France
                1. Jean-Michel Molina
                1. APHP Hôpital St Louis, France
                  1. Caroline Lascoux-Combe
                  1. APHP Hôpital St Louis, France
                    1. Jean-François Fléjou
                    1. APHP Hôpital St Antoine and UPMC University Paris, France
                      1. Pierre-Marie Girard
                      1. UPMC University Paris, APHP Hôpital St Antoine and Inserm UMR-S707 Paris, France

                        Abstract

                        Context: The METAVIR score, which is the most widely used score in France, was specifically elaborated and evaluated in chronic hepatitis C and has never been validated in HIV-HBV co-infected patients.

                        Aims: To validate the use of the METAVIR scoring system for activity and fibrosis on liver biopsies in co-infected HBV-HIV patients.

                        Methods: METAVIR scoring for activity and fibrosis was first conducted both original and virtual slides by one pathologist for comparison. Then 55 biopsies turned into virtual slides were scored by 3 pathologists independently.

                        Results: The scoring comparison between virtual slides and glass slides showed an almost perfect agreement for fibrosis (weighted Kappa (Kw) 0.8) and a substantial agreement for activity (Kw 0.68). The inter-observer agreement on virtual slides was moderate to almost perfect (Kw 0.52 to 0.84) for fibrosis and was dependant on the couple of pathologists considered. The best agreement was obtained in scoring advanced fibrosis and cirrhosis versus significant fibrosis versus no or mild fibrosis (Kw 0.70 to 0.84). The agreement for cirrhosis was rated moderate to substantial (Kw 0.54 to 0.79). Agreement for activity was substantial (Kw 0.66 to 0.8).

                        Conclusions: This study validates the use of virtual slide technology to assess fibrosis and activity on liver biopsies. It also validates the use of the METAVIR score in co-infected HBV-HIV patients and illustrates the challenges in establishing the histological diagnosis of cirrhosis in the HIV-HBV context.

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