Article Text

other Versions

PDF
Clinical Evaluation of NucliSENS Magnetic Extraction and NucliSENS Analytic Specific Reagents for the Real Time Detection of Respiratory Syncytial Virus (RSV) in Pediatric Respiratory Specimens
  1. Ryhana Manji (rmanji{at}nshs.edu)
  1. North Shore-LIJ Health System Labs, United States
    1. Madhavi Lotlikar (mlotlika{at}nshs.edu)
    1. North Shore-LIJ Health System Labs, United States
      1. Fan Zhang (fzhang{at}nshs.edu)
      1. North Shore-LIJ Health System Labs, United States
        1. Christine Ginocchio (cginocch{at}nshs.edu)
        1. North Shore-LIJ Health System Labs, United States

          Abstract

          Aims: To evaluate the combination of NucliSENS magnetic extraction and NucliSENS analytic specific reagents (bioMérieux, Marcy L’Etoile, France) for the detection of respiratory syncytial virus (RSV) from a variety of respiratory samples.

          Methods: Nucleic acids (NA) from pediatric samples (n=603) and a RSV specific inhibition control (R-IC) were co-extracted using the miniMAG and/or the easyMAG. Nucleic acid sequenced based amplification (NASBA) and molecular beacon detection of RSV and R-IC were performed using NucliSENS ASRs (NRSVA) and the NucliSENS EasyQ Analyzer. NRSVA results were compared to R-Mix culture and direct fluorescent antibody detection (DFA).

          Results: NRSVA analytical specificity was 100%. NRSVA limit of detection was 5-20 RNA copies/reaction. Pre discordant analysis, sensitivity, specificity, PPV and NPV were, respectively, for R-Mix (64.7%, 100%, 100%, 94.5%); DFA (98.8%, 99.0%, 94.4%, 99.8%); NRSVA (94.1%, 95%, 75.5%, 99%). After discordant analysis, sensitivity, specificity, PPV and NPV were, respectively, for R-Mix (56.7%, 100%, 100%, 92.3%); DFA (87.6%, 99.2%, 95.5%, 97.7%); NRSVA (93.8%, 97%, 85.9%, 98.8%). RSV was detected in 17.8% of the samples and in 7 co-infections. Children with proven RSV infection, compared to children without a pathogen identified, had shorter median hospitalization stays (2 days versus 3 days, P=0.035), used less antibiotics (54% versus 69%) and shorter durations of antibiotic therapy (6.2 days versus 9.3 days, P=0.021), respectively.

          Conclusions: NRSVA is sensitive and specific for RSV detection in respiratory samples. The R-IC monitored the test process, including NA extraction, target amplification and detection. The rapid detection of respiratory pathogens can foster appropriate patient management.

          Statistics from Altmetric.com

          Request permissions

          If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.