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Pitfalls in the use of multicolour flow cytometry in haematology
  1. Ulrika Johansson1,
  2. Marion Macey2
  1. 1Haematology Department, University Hospital's Bristol NHS Foundation Trust, Bristol, UK
  2. 2Department of Haematology, Bart's and The Royal London NHS Trust, London, UK
  1. Correspondence to Marion Macey, Department of Haematology, Bart's and The London NHS Trust, London, UK; marion.macey{at}


Multicolour flow cytometry in haematology has developed considerably in recent years. The ability to analyse eight or more colours of fluorescence on millions of cells in a matter of minutes has enabled the provision of rapid and reliable measures of minimal residual disease for clinicians. The use of multicolour analysis has also enabled more specific characterisation of presenting leukaemias and lymphomas. However, there has not been a concomitant increase in the knowledge and experience of the flow cytometrists to deal with certain problems associated with this more complex analysis.

  • Flow cytometry
  • haematology-oncology diagnostics

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  • Competing interests None.

  • Provenance and peer review Commissioned; not externally peer reviewed.

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