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Lymphoid aggregates may contribute to the migration and epithelial commitment of bone marrow-derived cells in colonic mucosa
  1. Gábor Valcz1,
  2. Tibor Krenács2,
  3. Ferenc Sipos1,
  4. Árpád V Patai1,
  5. Barnabás Wichmann1,
  6. Katalin Leiszter1,
  7. Kinga Tóth1,
  8. Zsófia Balogh2,
  9. Annamária Csizmadia2,
  10. Krisztina Hagymási1,
  11. Tamás Masszi3,
  12. Béla Molnár1,4,
  13. Zsolt Tulassay1,4
  1. 12nd Department of Internal Medicine, Semmelweis University, Budapest, Hungary
  2. 21st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
  3. 3St. István and St. László Hospital of Budapest, Hungary
  4. 4Molecular Medicine Research Unit, Hungarian Academy of Sciences, Budapest, Hungary
  1. Correspondence to Gábor Valcz, Cell Analysis Laboratory, 2nd Department of Internal Medicine, Semmelweis University, Budapest, Hungary; valczg{at}yahoo.com

Abstract

Aims Colonic inflammation is followed by regeneration supported by bone marrow-derived cells (BMDCs) including multipotent cells. They migrate to the colonic epithelial layer and may transdifferentiate into epithelial-like cells or keep their stem cell characteristics and produce progenies. The aim was to study the role of lymphoid aggregates in the migration and transition of BMDCs in both healthy colons and non-specific colitis (NSC).

Methods Samples of normal colon (n=5) and NSC (n=5) from female patients who were initially transplanted with male bone marrow were studied. After detecting XY chromosomes using fluorescent in situ hybridisation, tissue sections were digitalised, the coverslips were eliminated and the samples were double stained for CD45 and cytokeratin with immunofluorescence. Then CDX2 expression, as a sign of intestinal epithelial commitment of Musashi-1+ stromal BMDCs, was also tested with both immunoperoxidase and parallel immunofluorescence stainings. The slides were digitalised again and analysed simultaneously.

Results A significant increase in intraepithelial CD45–BMDCs was found in regions adjacent to lymphoid aggregates (median: 1.01) compared with healthy epithelial regions (median: 0.0175) or NSC (median: 0.04) samples. The stromal Musashi-1+ cells were positive for CDX2 as well, as a sign of epithelial differentiation. The CDX2+ cells bearing the Y chromosome proved the epithelial commitment of several stromal BMDCs.

Conclusion Elevated number of intraepithelial CD45−BMDCs at lymphoid aggregates suggests that BMDCs play a role in epithelial regeneration and that lymphoid aggregates serve as their migration route.

  • Bone marrow-derived cells
  • epithelial regeneration
  • epithelial commitment
  • stem cell transplants
  • gastroenterology
  • inflammatory bowel disease
  • colorectal cancer
  • gall bladder
  • oncogenes
  • P53
  • pancreas

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Footnotes

  • Competing interests None.

  • Patient consent Obtained.

  • Provenance and Peer review Not commissioned; externally peer reviewed.

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