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Correlation between expression of cellular retinol-binding protein 1 and its methylation status in larynx cancer
  1. Raúl Peralta1,
  2. Alejandra Valdivia1,
  3. Isabel Alvarado-Cabrero2,
  4. Francisco Gallegos3,
  5. Teresa Apresa1,4,
  6. Dulce Hernández4,
  7. Mónica Mendoza1,
  8. Pablo Romero1,
  9. Lucero Paniagua1,
  10. Miguel Ibáñez5,
  11. Lourdes Cabrera6,
  12. Mauricio Salcedo1
  1. 1Laboratorio de Oncogenomica, UIMEO, Hospital de Oncología, CMN-SXXI, IMSS, México, Distrito Federal, Mexico
  2. 2Departamento de Patología, Hospital de Oncología, CMN-SXXI, IMSS, México, Distrito Federal, Mexico
  3. 3Servicio de Cabeza y Cuello, Hospital de Oncología, CMN-SXXI, IMSS, México, Distrito Federal, Mexico
  4. 4Sección de Epidemiología, Hospital de Oncología, CMN-SXXI, IMSS, México, Distrito Federal, Mexico
  5. 5Departamento de Bioquímica, ENCB-IPN, México, Distrito Federal, Mexico
  6. 6Departamento de Patología, Hospital de Especialidades, CMN-SXXI, IMSS, México, Distrito Federal, Mexico
  1. Correspondence to Dr Mauricio Salcedo, Laboratorio de Oncogenómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI, IMSS, Av. Cuauhtémoc 330, Col. Doctores, México DF 06720, Mexico; maosal89{at}


Aims The authors have previously reported that cellular retinol-binding protein 1 (CRBP1) gene gain and its expression correlated significantly with survival in laryngeal carcinoma patients. The authors hypothesised that inactivation of the CRBP1 gene through CpG methylation is associated with patient status and gene expression. In this work, the authors determine the expression and methylation status of the CRBP1 gene and its correlation with clinical variables of laryngeal carcinoma patients.

Methods The CRBP1 gene methylation promoter was assessed by methylation specific PCR analysis in tissue samples from larynx cancer specimens and its expression was assessed by immunohistochemistry on paraffin embedded tissue using tissue microarray. The results were then compared with the clinical pathological variables and outcome measures. The study included 46 samples from patients with non-metastatic squamous cell carcinoma of the larynx without any previous oncological treatments.

Results Lack of CRBP1 expression was seen in 17 of the 46 laryngeal carcinoma samples, while the remaining 29 samples showed increased expression. Significant associations were found between CRBP1 expression and methylation and patient status. There was a tendency for association in all clinical stages of the disease. CRBP1 gene expression and its unmethylated promoter correlated significantly with survival (p<0.05).

Conclusions An early event of larynx cancer could be CRBP1 expression related to unmethylation of the promoter region. These features could also be associated with good response and survival. The authors hypothesised that increased expression and unmethylated promoter of the CRBP1 gene could be considered as markers for larynx cancer.

  • Cancer genetics
  • cancer research
  • cervical cancer
  • laryngeal cancer
  • cancer
  • carcinoma
  • larynx

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  • Funding This work was partially supported by grants 69719 and 87244, Fondos Sectoriales de Salud, CONACYT, México. RP, MM and AV were recipients of CONACYT fellowship.

  • Competing interests None.

  • Patient consent Obtained.

  • Ethics approval Local committee of ethics of the Mexican Institute of Social Security (IMSS).

  • Provenance and peer review Not commissioned; externally peer reviewed.

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