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Effect of EpCAM, CD44, CD133 and CD166 expression on patient survival in tumours of the ampulla of Vater
  1. Salvatore Piscuoglio1,
  2. Frank S Lehmann2,
  3. Inti Zlobec1,
  4. Luigi Tornillo1,
  5. Wolfgang Dietmaier3,
  6. Arndt Hartmann4,
  7. Peter H Wünsch5,
  8. Fausto Sessa6,
  9. Petra Rümmele3,
  10. Daniel Baumhoer1,
  11. Luigi M Terracciano1
  1. 1Institute of Pathology, University Hospital of Basel, Basel, Switzerland
  2. 2Department of Gastroenterology and Hepatology, University Hospital of Basel, Basel, Switzerland
  3. 3Department of Pathology, University Hospital of Regensburg, Regensburg, Germany
  4. 4University Hospital of Erlangen, Erlangen, Germany
  5. 5Municipal Hospital of Nürnberg, Nürnberg, Germany
  6. 6Anatomic Pathology Unit, University of Insubria, Varese, Italy
  1. Correspondence to Prof Luigi M Terracciano, Institute of Pathology, University Hospital of Basel, Schönbeinstrasse 40, 4003 Basel, Switzerland; lterracciano{at}uhbs.ch

Abstract

Background Carcinomas of the Vaterian system are rare and presumably arise from pre-existing adenomas. According to the cancer stem cell (CSC) hypothesis, only a small subset of tumor cells has the ability to initiate and develop tumor growth. In colorectal cancer, CD44, CD133, CD166 and EpCAM have been proposed to represent CSC marker proteins and their expression has been shown to correlate with patient survival.

Aims To evaluate a potential role of these CSC proteins in tumors of the ampulla of Vater, we investigated their expression in 175 carcinoma, 111 adenoma and 152 normal mucosa specimens arranged in a Tissue Microarray format.

Materials and methods Membranous immunoreactivity for each protein marker was scored semi-quantitatively by evaluating the number of positive tumor cells over the total number of tumor cells. Median protein expression levels were used as cut-off scores to define protein marker positivity. Clinical data including survival time were obtained by retrospective analysis of medical records, tumor registries or direct contact.

Results The expression of all evaluated marker proteins differed significantly between normal mucosa, adenoma and carcinoma samples. In all markers, we found a tendency towards more constant expression from normal to neoplastic tissue. EpCAM expression was significantly correlated with better patient survival. The increased expression of CD44s, CD166 and CD133 from normal mucosa samples to adenoma and carcinoma was linked to tumor progression. However, there was no statistically significant correlation with survival.

Conclusion Our findings indicate, that in ampullary carcinomas, loss of expression of EpCAM may be linked to a more aggressive tumor phenotype.

  • CSC
  • ampulla of Vater
  • cancer
  • survival
  • EpCAM
  • colorectal cancer
  • gastrointestinal disease
  • tumour markers
  • rectal cancer
  • oncogenes
  • molecular pathology
  • bladder
  • renal cancer
  • cancer genetics
  • genitourinary pathology
  • GI neoplasms
  • gut pathology
  • microarray
  • hepatitis
  • liver disease
  • liver

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Footnotes

  • Salvatore Piscuoglio and Frank S Lehmann are equally contributed to this work.

  • Competing interests None.

  • Patient consent If yes, who signed it?

  • Ethics approval Ethische Kommission Beider Basel.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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