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Expression of p53, p21 and cyclin D1 in penile cancer: p53 predicts poor prognosis
  1. Sven Gunia1,
  2. Christoph Kakies2,
  3. Andreas Erbersdobler2,
  4. Oliver W Hakenberg3,
  5. Stefan Koch1,
  6. Matthias May4
  1. 1Institute of Pathology, HELIOS Clinic Bad Saarow, Germany
  2. 2Institute of Pathology, University of Rostock, Germany
  3. 3Department of Urology, University of Rostock, Germany
  4. 4Department of Urology, St Elisabeth Clinic, Straubing, Germany
  1. Correspondence to Sven Gunia, Institute of Pathology, HELIOS Clinic Bad Saarow, Charité-University Medicine Teaching Hospital, Pieskower Straße 33, 15526 Bad Saarow, Germany; sven.gunia{at}helios-kliniken.de

Abstract

Aims To evaluate the role of p53, p21 and cyclin D1 expression in patients with penile cancer (PC).

Methods Paraffin-embedded tissues from PC specimens from six pathology departments were subjected to a central histopathological review performed by one pathologist. The tissue microarray technique was used for immunostaining which was evaluated by two independent pathologists and correlated with cancer-specific survival (CSS). κ-statistics were used to assess interobserver variability. Uni- and multivariable Cox proportional hazards analysis was applied to assess the independent effects of several prognostic factors on CSS over a median of 32 months (IQR 6–66 months).

Results Specimens and clinical data from 110 men treated surgically for primary PC were collected. p53 staining was positive in 30 and negative in 62 specimens. κ-statistics showed substantial interobserver reproducibility of p53 staining evaluation (κ=0.73; p<0.001). The 5-year CSS rate for the entire study cohort was 74%. Five-year CSS was 84% in p53-negative and 51% in p53-positive PC patients (p=0.003). Multivariable analysis showed p53 (HR=3.20; p=0.041) and pT-stage (HR=4.29; p<0.001) as independent significant prognostic factors for CSS. Cyclin D1 and p21 expression were not correlated with survival. However, incorporating p21 into a multivariable Cox model did contribute to improved model quality for predicting CSS.

Conclusions In patients with PC, the expression of p53 in the primary tumour specimen can be reproducibly assessed and is negatively associated with cancer specific survival.

  • Bladder
  • cancer
  • histopathology
  • colorectal cancer
  • gall bladder
  • oncogenes
  • p53
  • pancreas

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Footnotes

  • Competing interests None declared.

  • Ethics approval The study was approved by the Medical Ethics Committee of the federal state Brandenburg (Landesärztekammer Brandenburg, LÄKB), reference number 27365/2011.

  • Contributors All authors named significantly contributed to the present study.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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