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PAX8 is expressed in the majority of renal epithelial neoplasms: an immunohistochemical study of 223 cases using a mouse monoclonal antibody
  1. Yingchuan Hu1,
  2. Arndt Hartmann2,
  3. Christine Stoehr2,
  4. Shaobo Zhang1,
  5. Mingsheng Wang1,
  6. David Tacha3,
  7. Rodolfo Montironi4,
  8. Antonio Lopez-Beltran5,
  9. Liang Cheng1
  1. 1Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA
  2. 2Department of Pathology, University of Erlangen, Erlangen, Germany
  3. 3Biocare Medical, Concord, California, USA
  4. 4Department of Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of the Marche Region (Ancona), Ancona, Italy
  5. 5Department of Pathology, Cordoba University, Cordoba, Spain
  1. Correspondence to Dr Liang Cheng, Department of Pathology, Indiana University School of Medicine, 350 West 11th Street, IU Health Pathology Laboratory, Room 4010, Indianapolis, IN 46202, USA; liang_cheng{at}yahoo.com

Abstract

Aims PAX8 is a cell lineage-specific transcription factor which plays a crucial role in the organogenesis of the kidney, thyroid gland and Müllerian duct. A previous study showed that PAX8 is a specific and sensitive marker for both renal and ovarian carcinomas. The purpose of this study is to investigate PAX8 expression using a new monoclonal PAX8 antibody in a larger number of renal epithelial neoplasms including clear cell renal cell carcinoma, papillary renal cell carcinoma, chromophobe renal cell carcinoma and renal oncocytoma.

Methods PAX8 immunohistochemical staining was performed on tissue microarrays containing 84 cases of clear cell renal cell carcinoma, 66 cases of chromophobe renal cell carcinoma, 57 cases of papillary renal cell carcinoma and 16 cases of renal oncocytoma.

Results PAX8 expression was detected in 93% (78/84) of cases of clear cell renal cell carcinoma, 80% (53/66) of cases of chromophobe renal cell carcinoma, 95% (54/57) of cases of papillary renal cell carcinoma and 94% (15/16) of cases of renal oncocytoma.

Conclusions PAX8 is expressed in the majority of renal epithelial neoplasms including renal cell carcinomas and oncocytomas and the monoclonal PAX8 antibody is more sensitive than polyclonal antibody to detect chromophobe renal cell carcinoma. These results showed that PAX8 is a valuable marker for nephric neoplasms.

  • PAX8
  • immunohistochemistry
  • renal cell carcinoma
  • renal oncocytoma
  • bladder
  • renal cancer
  • cancer genetics
  • molecular pathology
  • genitourinary pathology
  • brain tumours
  • cancer stem cells
  • uropathology
  • morphometry
  • prostate
  • cell cycle regulation
  • surgical pathology
  • urinary tract tumours
  • urogenital pathology
  • evidence based pathology
  • tumour markers

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Footnotes

  • Competing interests None.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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