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Expression of claudins 7 and 18 in pancreatic ductal adenocarcinoma: association with features of differentiation
  1. Y Soini1,
  2. A Takasawa2,
  3. M Eskelinen3,
  4. P Juvonen3,
  5. V Kärjä4,
  6. T Hasegawa5,
  7. M Murata2,
  8. S Tanaka2,
  9. T Kojima2,
  10. N Sawada2
  1. 1Department of Pathology and Forensic Medicine, Institute of Clinical Medicine, School of Medicine, University of Eastern Finland, Cancer Center of Eastern Finland, Kuopio, Finland
  2. 2Department of Pathology, School of Medicine, Sapporo Medical University, Sapporo, Japan
  3. 3Department of Surgery, Kuopio University Hospital and School of Medicine, University of Eastern Finland, Kuopio, Finland
  4. 4Department of Clinical Pathology, Diagnostic Imaging Centre, Kuopio University Hospital, Kuopio, Finland
  5. 5Department of Surgical Pathology, School of Medicine, Sapporo Medical University, Sapporo, Japan
  1. Correspondence to Dr Ylermi Soini, Department of Pathology and Forensic Medicine, School of Medicine, University of Eastern Finland, PO Box 1627, FI-70211 Kuopio, Finland; ylermi.soini{at}uef.fi

Abstract

Aim This study was undertaken to evaluate the expression of claudins 7 and 18 in pancreatic ductal adenocarcinoma.

Methods and results Material tested included 111 operated samples and 47 additional biopsy samples consisting of 26 cases of pancreatitis, 3 cases of pancreatic intraepithelial neoplasia and 18 ductal adenocarcinomas. Samples were stained with antibodies to claudins 7 and 18 and analysed for membranous and cytoplasmic expression. Membrane bound claudin 7 and 18 expression was detected in 62 of 105 (59%) and 78 of 111 (70%) cases, respectively. Membrane bound claudin 7 and 18 were associated with large or intermediate neoplastic ducts (p=0.01, p=0.002, respectively). Well differentiated pancreatic adenocarcinomas displayed more cases with membrane bound claudin 7 or 18 immunopositivity (p=0.003, p=0.03, respectively). All pancreatic intraepithelial neoplasias studied expressed membrane bound claudin 18. Membrane bound claudin 7 or 18 positivity was not associated with survival (p=0.17, p=0.98). In the biopsy cases membrane bound claudin 18 had 100% specificity and 51% sensitivity for a tumour marker.

Conclusion Claudin 7 and 18 expression is related to gland size of neoplastic cells and is especially found in tumours with intermediate and large ducts and well differentiated tumours. Membrane bound claudin 18, when present, is a useful marker for diagnosis of pancreatic cancer. Claudins 7 and 18 were not associated with patient survival or spread of tumours.

  • Pancreas
  • cancer
  • claudin
  • tight junction
  • cancer research
  • soft tissue tumours
  • soft tissue
  • breast cancer
  • pleura
  • bone tumours
  • soft tissue tumours
  • bone tumour pathology
  • cell biology
  • molecular biology

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Footnotes

  • Funding This study was supported by the Paulo Foundation and by the Finnish Anti-Tuberculosis Association.

  • Competing interests None.

  • Ethics approval Ethics approval was provided by the ethical board of the Northern Kuopio Hospital District.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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