Article Text

other Versions

PDF
Cytotoxicity of gemcitabine enhanced by polyphenolics from Aronia melanocarpa in pancreatic cancer cell line AsPC-1
  1. Noor Azela Abdullah Thani1,
  2. Sholeh Keshavarz1,
  3. Bashir A Lwaleed2,
  4. Alan J Cooper3,
  5. Harcharan K Rooprai4
  1. 1School of Science and Technology, Middlesex University, The Burroughs, London, UK
  2. 2Faculty of Health Sciences, University of Southampton, Southampton, Hampshire, UK
  3. 3School of Pharmacy and Biomedical Sciences, Portsmouth University, Portsmouth, Hampshire, UK
  4. 4Department of Neurosurgery, King's College Hospital, London, UK
  1. Correspondence to Dr Bashir A Lwaleed, Faculty of Health Sciences, University of Southampton, South Academic and Pathology Block (MP 11), Southampton General Hospital, Tremona Road, Southampton, Hampshire SO16 6YD, UK; bashir{at}soton.ac.uk

Abstract

Aims Extending work with brain tumours, the hypothesis that micronutrients may usefully augment anticancer regimens, chokeberry (Aronia melanocarpa) extract was tested to establish whether it has pro-apoptotic effects in AsPC-1, an established human pancreatic cell line, and whether it potentiates cytotoxicity in combination with gemcitabine. Pancreatic cancer was chosen as a target, as its prognosis remains dismal despite advances in therapy.

Methods An MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay was used to assess the growth of the single pancreatic cancer cell line AsPC-1, alone and in comparison or combination with gemcitabine. This was backed up by flow cytometric DRAQ7 cell viability analysis. TUNEL assays were also carried out to investigate pro-apoptotic properties as responsible for the effects of chokeberry extract.

Results Chokeberry extract alone and its IC75 value (1 µg/mL) in combination with gemcitabine were used to assess the growth of the AsPC-1 cell line. Gemcitabine in combination with chokeberry extract was more effective than gemcitabine alone. TUNEL assays showed apoptosis to be a mechanism occurring at 1 µg/mL concentration of chokeberry, with apoptotic bodies detected by both colourimetric and fluorometric methods.

Conclusions The implication of this study, using single cancer cell line, is that chemotherapy (at least with gemcitabine) might be usefully augmented with the use of micronutrients such as chokeberry extract.

  • antioxidants
  • CANCER RESEARCH
  • PANCREATIC CANCER
  • TUMOUR BIOLOGY
  • APOPTOSIS

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.