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Correlation between DPYD gene variation and KRAS wild type status in colorectal cancer
  1. Britta Kleist1,
  2. Marcel Kempa2,
  3. Thuja Meurer2,
  4. Micaela Poetsch2
  1. 1Department of Pathology, Southern Hospital Trust, Kristiansand, Norway
  2. 2Institute of Legal Medicine, University Hospital Essen, Essen, Germany
  1. Correspondence to Dr Britta Kleist, Department of Pathology, Southern Hospital Trust, Soerlandet sykehus HF, Kristiansand 4604, Norway; britta.kleist{at}sshf.no

Abstract

Aims Failure and side effects of combined cytotoxic therapy are challenges in the treatment of metastatic colorectal cancer (CRC). DPYD gene variations can potentially predict toxicity to 5-fluorouracil (FU)-based therapy and KRAS-, NRAS-, BRAF-, PIK3CA-wild type status is a known prerequisite for epidermal growth factor receptor (EGFR) inhibitor therapy. This study was performed to search for a possible link between these therapeutic markers.

Methods The DPYD gene variations c.496A>G, c.1679T>G, c.2846A>T and KRAS/NRAS/BRAF/PIK3CA mutational status were determined in non-neoplastic, primary CRC and metastatic CRC tissue from 115 patients.

Results The polymorphism c.496A>G was the DPYD gene variant with the highest detection rate (12.9%), occurred predominantly in females (86.7%, p=0.0044) and was exclusively seen in KRAS wild type primary CRC (15/65 (23.1%) vs 0/51 (0%) in KRAS-mutated primary CRC, respectively, p=0.0001).

Conclusions This genetic profile could define a patient group requiring alternative combined therapeutic approaches. Global testing of large patient cohorts is necessary to prove this concept.

  • CANCER GENETICS
  • COLORECTAL CANCER
  • DIAGNOSTICS

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