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The retinoblastoma gene family pRb/p105, p107, pRb2/p130 and simian virus-40 large T-antigen in human mesotheliomas

Nature Medicine volume 3, pages 913–916 (1997)Cite this article

Abstract

The oncoprotein of simian virus-40, SV40 large T-antigen (Tag), is reported to target and to inactivate growth suppressive proteins such as the retinoblastoma family1–3 and p53 (ref. 4, 5), leading to transformation of human cell lines in vitro, tumor production in rodents6, and detection of Tag in several human cancers including mesotheliomas7,8. The retinoblastoma family contains three members, pRb, p107 and pRb2/p130 (ref. 9), that are phosphorylated in a cell cycle-dependent manner10,11, have cell growth suppressive properties12 and bind to specific members of the E2F family and various cyclins13. Even though mesotheliomas are among the most aggressive human cancers, alterations of important cell-cycle “controllers,” such as the Rb family genes, have never been reported in these tumors. We found the presence of SV40-like sequences in 86% of 35 archival specimens of mesothelioma. We also demonstrated that SV40 Tag, isolated from frozen biopsies of human mesothelioma, binds each of the retinoblastoma family proteins, pRb, p107 and pRb2/p130, in four of four specimens. We propose that the tumorigenic potential of SV40 Tag in some human mesotheliomas may arise from its ability to interact with and thereby inactivate several tumor and/or growth suppressive proteins.

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Author notes

  1. Antonio Giordano: Correspondence should be addressed to A.G.

  2. Antonio De Luca, Alfonso Baldi and Vincenzo Esposito: The first three authors contributed equally to this work

Authors and Affiliations

  1. Department of Pathology, Anatomy & Cell Biology and Sbarro Institute for Cancer Research and Molecular Medicine, Jefferson Medical College, 1020 Locust Street, Philadelphia, Pennsylvania, 19107, USA

    Antonio De Luca, Alfonso Baldi, Vincenzo Esposito, Candace M. Howard, Luigi Bagella & Antonio Giordano

  2. Istituto di Tisiologia e Malattie Respiratorie “S. Marcatili,” Facoltà di Medicina, II Università di Napoli, Via L. Bianchi, Napoli, 80122, Italy

    Vincenzo Esposito & Mario Caputi

  3. Cardinal Bemardin Cancer Center, Cancer Immunology Program, Department of Pathology, Loyola University, 2160 South First Avenue, Chicago, Maywood, Illinois, 60153, USA

    Paoloa Rizzo & Michele Carbone

  4. Karmanos Cancer Institute, Wayne State University, 3990 John Road, Detroit, Michigan, 48201, USA

    Harvey I. Pass

  5. Istituto di Anatomia Patologica, Facoltà di Medicina, II Università di Napoli, Via L. Armanni, Napoli, 80122, Italy

    Giovan Giacomo Giordano & Feliciano Baldi

Authors
  1. Antonio De Luca
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  2. Alfonso Baldi
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  7. Mario Caputi
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  8. Harvey I. Pass
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  9. Giovan Giacomo Giordano
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  10. Feliciano Baldi
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  11. Michele Carbone
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  12. Antonio Giordano
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De Luca, A., Baldi, A., Esposito, V. et al. The retinoblastoma gene family pRb/p105, p107, pRb2/p130 and simian virus-40 large T-antigen in human mesotheliomas. Nat Med 3, 913–916 (1997). https://doi.org/10.1038/nm0897-913

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