Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Technical Report
  • Published:

Umbilical cord blood T lymphocytes are induced to apoptosis after being allo-primed in vitro

Abstract

In this study, the immunity of umbilical cord blood (UCB) T lymphocytes against allo-antigens was investigated by a standard MLC. No significant difference, between the UCB T cells or peripheral blood (PB) mature T cells, was observed in the primary responses (stimulation index (SI), 51.8 ± 14.8 and 46.5 ± 15.0, respectively). In contrast, in the secondary response, the SI obtained with the CD4 T cells from UCB decreased dramatically (16.3 ± 6.4), while it increased with the CD4 T cells from PB (118.5 ± 21.7). UCB (CD4 and CD8) T cells separately showed much higher frequencies of apoptosis after a primary allo-priming, compared with PB CD4 and CD8 T cells (CD4, UCB 30.5% vs PB 0.8%; CD8, UCB 32% vs PB 1.3%). The higher apoptotic level of the UCB CD4 T cells was confirmed by a second, ELISA-based, Tunel assay (OD values, UCB CD4 1.93 ± 0.31 vs PB CD4 0.59 0.9; P < 0.01). those apoptotic steps were not attributed to the amount of cytokine (il-2, 4 and ifn-gamma) production, which was found to be similar in both cases. in conclusion, ucb lymphocytes are much more likely to be induced to apoptosis by allo-priming than adult lymphocytes. this supports their possible, successful engraftment across barriers of hla incompatibility.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hagihara, M., Chargui, J., Gansuvd, B. et al. Umbilical cord blood T lymphocytes are induced to apoptosis after being allo-primed in vitro. Bone Marrow Transplant 24, 1229–1233 (1999). https://doi.org/10.1038/sj.bmt.1702050

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.bmt.1702050

Keywords

This article is cited by

Search

Quick links