Abstract
Peutz–Jeghers syndrome (PJS, MIM175200) is an autosomal-dominant inherited disorder characterised by multiple gastrointestinal hamartomatous polyps, melanin spots of the oral mucosa and digits, and an increased risk for various neoplasms. The PJS results from germline alterations of the STK11/LKB1 tumour suppressor gene, located on 19p13.3, and encoding a serine/threonine kinase. The detection of STK11 germline mutations, in only 50–70% of PJS families, has suggested a genetic heterogeneity of the disease. We report the case of a family with typical features of PJS, including gastrointestinal hamartomatous, breast cancers and melanin spots of the oral mucosa. Quantitative multiplex PCR of short fluorescent fragments (QMPSF) of the 19p13 region allowed us to identify an approximately 250 kb heterozygous deletion removing entirely the STK11 locus. This report, which constitutes the first description of a complete germline deletion of STK11, shows that the presence of such large genomic deletions should be considered in PJS families without detectable point mutations of STK11.
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Introduction
Peutz–Jeghers syndrome (PJS) is a rare autosomal-dominant disease associated with predisposition to benign and malignant tumours. The incidence has been estimated as one in 100 000–200 000 births.1,2,3 Patients present mucocutaneous pigmented macules, especially on the lips, buccal mucosa and digits, diffuse gastrointestinal hamartomatous polyposis and an increased risk of early-onset cancers, such as gastrointestinal carcinoma, breast cancer, genital tract tumours and pancreatic cancer.4 The gene involved in PJS was mapped to chromosome 19p13.35,6 and was shown to be STK11/LKB1.7,8 The STK11 gene contains 10 exons spanning 23 kb, and encodes an ubiquitously expressed 433 amino-acid serine threonine–kinase, which might be involved in G1 cell cycle arrest,9 p53-dependent apoptosis pathway,10 vascular endothelial growth factor regulation,11 and in the Wnt – -beta catenin signalling pathway.12 Most of the STK11 mutations detected in PJS patients result in truncated proteins with incomplete catalytic domains without kinase activity.7,8,13,14 PJS represents, therefore, the first cancer susceptibility syndrome resulting from germline inactivation of a kinase activity. The formation of hamartomatomas and cancers, in STK11 germline mutation carriers, results from the somatic inactivation of the wild-type allele, according to the Knudson model for tumour suppressor genes inactivation.5,15 Germline mutations of the STK11 gene can be detected in only 50–70% of PJS families7,16,17,18 and in 12–50% of individuals with sporadic PJS,16,19 which may reflect the involvement of other unidentified genes in PJS. Indeed, PJS families, not linked to chromosome 19p13.3, have already been reported.20,21 The alternative hypothesis, which might explain the absence of detectable STK11 germline mutations in some PJS families, is the existence of alterations, such as genomic rearrangements, which are missed by conventional screening methods.
We report here, for the first time, the detection of a complete genomic deletion of the STK11 locus in a family with PJS.
Patients and methods
Patients
The proband, born in 1987, was a male from nonconsanguineous parents and the youngest of three siblings. He was initially referred to our department of genetics for learning disabilities and scoliosis. Examination revealed ‘Café-au-lait’ spots and led to the diagnosis of neurofibromatosis type 1, which was confirmed by the presence of Lisch nodules. At the age of 13 years, he presented an occlusive syndrome with rectal prolapse in relation with a large (5 × 4 cm) sigmoid polyp. The existence of a lentiginosis of the inferior lip led us to suspect a PJS, which was confirmed by pathological examination of the polyp. The father presented ‘café au lait’ spots and a thoracic neurofibroma, which was consistent with the paternal origin of neurofibromatosis type 1. The mother of the index case had developed, (i) at the age of 43 years, recurrent hamartomatous polyps and a colorectal cancer revealed by bleeding, (ii) at the age of 44 years, a right-breast carcinoma and, (iii) at the age of 45 years, a left-breast carcinoma. The maternal uncle, presented hamartomatous polyps of the colon and colorectal cancer at the age of 43 years. Re-examination of the polyps developed in the mother and the maternal uncle, after the diagnosis of PJS in the index case, confirmed that these polyps were consistent with the diagnosis of PJS. Therefore, the proband had simultaneously an NF1 from paternal origin and a PJS from maternal origin.
Microsatellite analysis
The centromeric and telomeric microsatellite markers D19S886 and D19S883, respectively, located 180 kb upstream and 234 kb downstream of STK11, were PCR amplified from genomic DNA using dye-labelled primers and analysed on an automated sequencer (Applied Biosystems) using the Gene scanner Model 672 Fluorescent Fragment Analyzer (Applied Biosystems).
Fluorescence. in situ hybridization (FISH) analysis
The RP11-75H6 BAC, obtained from the University of Bari, Italy (http://www.biologia.uniba.it/rmc), was labelled using the Nick translation Reagent Kit (Vysis) and fluorescein-labelled dUTPs (Vysis). Metaphase chromosome spreads and probe were denatured at 72°C for 1 min, then hybridized at 37°C overnight. Chromosome counterstaining was performed with 0.1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) in antifade solution. Slides were visualized on a Nikon-Microphot-FXA microscope and the images were captured using the ISIS digital FISH imaging system (Metasystem).
Quantitative multiplex PCR of short fluorescent fragments (QMPSF)
Short exonic fragments, corresponding to the 10 exons of STK11 and to the exon 13 of HMBS/PBGD located on chromosome 11, used as control, were simultaneously amplified in a first QMPSF. To examine the extent of the deletion, we designed a second QMPSF including exon 1 of WDR18, exon 5 of MIDN, exon 18 of DAZAP1 and exon 18 of MLH1, used as control. Sequences of primers are indicated in Table 1.
Results
The remarkable association of NF1 and PJS features in the same patient led us initially to test the involvement of the STK11 gene within this family. Therefore, we analysed first the segregation of the D19S886 and D19S883 microsatellite markers, surrounding the STK11 locus, in the index case, the mother and maternal uncle whose clinical presentation was consistent with PJS. Unexpectedly, this analysis showed an absence of maternal contribution of D19S886 in the proband, suggesting therefore a 19p13.3 heterozygous deletion inherited from his affected mother (data not shown). Chromosome analysis performed on cultured peripheral blood lymphocytes from the proband, using standard R-banding, revealed no alteration at the 400 band level. FISH analysis, performed with a genomic probe prepared from the RP11-75H6 clone, revealed an asymmetric signal between the two chromosomes 19, which indicated that this BAC overlapped the deleted region (data not shown). In order to confirm the heterozygous deletion of STK11 and to map precisely the deletion, we used QMPSF, a method based on simultaneous amplification of multiple short sequences under quantitative conditions.22 QMPSF, performed in the proband, his mother and maternal uncle, confirmed the deletion and revealed that this deletion removed all the exons of STK11 (Figure 1a). In contrast, the telomeric WDR18, and centromeric DAZAP1 and MIDN genes were not deleted (Figure 1b).
Discussion
We document here for the first time, in a PJS family, using microsatellite, FISH and QMPSF analysis, a complete heterozygous deletion of the STK11 locus. Results from the microsatellite markers and QMPSF analysis allowed us to locate the telomeric breakpoint between WDR18 exon 1 and D19S886 and the centromeric breakpoint between STK11 exon 10 and MIDN exon 5 (Figure 2), and to estimate the size of this 19p13.3 deletion between 220 and 250 kb. According to the chromosome 19 draft sequence (http://www.ncbi.nlm.nih.gov/LocusLink), this deletion also removed, in addition to STK11, the GPX4, POLR2E, HA-1, CNN2 and ABCA7 genes encoding, respectively, the glutathion peroxydase-4, the subunit E of DNA-dependant polymerase II, a minor histocompatibility antigen, the calponin 2 and an ATP-binding cassette protein (Figure 2).
To date, approximately 100 different mutations of STK11 have been described in PJS patients (Human Gene Mutation Database: http://archive.uwcm.ac.uk/uwcm/mg/hgmd0.html). Most mutations are nucleotide substitutions and small insertions or deletions. At the present time, four partial genomic rearrangements of STK11 have been detected: a deletion of the end of intron 6 and the majority of exon 7,13 a complex rearrangement including a deletion of exons 4–5 and an inversion of exons 6–7 detected by long-distance PCR,8 a deletion of exons 2–7 and a part of exon 823 revealed by Protein Truncation Test and a 2 kb deletion detected by Southern blot.16 Our study shows that complete heterozygous rearrangements of STK11 also occur in PJS. PJS families linked to 19p13 and without detectable mutation of STK11 have already been described.7,16 The presence of such genomic rearrangements, which are missed by conventional screening methods, based on PCR amplification of each exon, should be therefore considered in PJS families without detectable alteration. The QMPSF method, which can detect heterozygous deletions affecting either a single or several exon(s), should allow to estimate the relative contribution of STK11 deletions in the aetiology of PJS.
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Acknowledgements
We are grateful to Mario Tosi for a critical review of the manuscript. This work was supported by l'Association pour la Recherche sur le Cancer.
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Meur, N., Martin, C., Saugier-Veber, P. et al. Complete germline deletion of the STK11 gene in a family with Peutz–Jeghers syndrome. Eur J Hum Genet 12, 415–418 (2004). https://doi.org/10.1038/sj.ejhg.5201155
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DOI: https://doi.org/10.1038/sj.ejhg.5201155
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