Chromosome 1 deletions in human neuroblastomas: generation and fine mapping of microclones from the distal 1p region

Genes Chromosomes Cancer. 1989 Sep;1(1):67-78. doi: 10.1002/gcc.2870010111.

Abstract

Human neuroblastomas show a high incidence of deletions in the distal region of the short arm of chromosome 1. In pursuit of a molecular analysis of these deletions, we have generated a microclone bank from microdissected 1p35-pter chromosomal fragments. To allow a rapid localization of the microclones, we have also generated a panel of (human x mouse) hybrid cell lines through microcell-mediated chromosome transfer. The hybrid cells contained different portions of the human chromosome 1 on a murine background. A total of 20 randomly chosen single or low-copy microclones were localized by Southern analysis on DNA of the hybrid panel: All probes were derived from chromosome I. Sixteen mapped in region 1p36.1-pter, two in 1p22-p36.1, and another two in 1cen-qter. The mapping of ten of these microclones was further refined by in situ hybridization. Cells of the neuroblastoma line GI-ME-N carry two types of chromosome 1, one cytogenetically normal and another with a translocation reported to be in 1p36.2, i.e., a t(1;?) (p36.2;?) marker. Using cell hybridization, we separated the two chromosome 1 types of GI-ME-N into different hybrid cell clones. Southern hybridization of three microclones from distal Ip to DNA of the hybrid cell clones revealed that the breakpoint in the translocated chromosome I was located in 1p36.1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage lambda
  • Blotting, Southern
  • Chromosome Deletion*
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 1*
  • Cloning, Molecular / methods
  • DNA, Neoplasm / genetics
  • Dissection / methods
  • Genetic Vectors
  • Humans
  • Hybrid Cells
  • Karyotyping
  • Metaphase / genetics
  • Moloney murine leukemia virus
  • Neuroblastoma / genetics*
  • Nucleic Acid Hybridization

Substances

  • DNA, Neoplasm