Glycogen synthase kinase 3 beta is identical to tau protein kinase I generating several epitopes of paired helical filaments

K Ishiguro; A Shiratsuchi; S Sato; A Omori; M Arioka; S Kobayashi; T Uchida; K Imahori

doi:10.1016/0014-5793(93)81066-9

Glycogen synthase kinase 3 beta is identical to tau protein kinase I generating several epitopes of paired helical filaments

FEBS Lett. 1993 Jul 5;325(3):167-72. doi: 10.1016/0014-5793(93)81066-9.

Authors

K Ishiguro¹, A Shiratsuchi, S Sato, A Omori, M Arioka, S Kobayashi, T Uchida, K Imahori

Affiliation

¹ Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.

PMID: 7686508
DOI: 10.1016/0014-5793(93)81066-9

Abstract

We previously reported that tau protein kinase I (TPKI) induced normal tau protein into a state of paired helical filaments (PHF); this is further confirmed here by immunoblot analysis using several antibodies. We also present the amino acid sequence of TPKI, which is identical to glycogen synthase kinase 3 beta (GSK3 beta). Moreover, we found that TPKI activity was inseparable from GSK3 activity throughout the purification procedure. These results indicate that TPKI is identical to GSK3 beta.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Calcium-Calmodulin-Dependent Protein Kinases
Cattle
DNA
Epitopes
Glycogen Synthase Kinase 3
Glycogen Synthase Kinases
Humans
Immunoblotting
Molecular Sequence Data
Protein Kinases / chemistry*
Protein Kinases / immunology
Protein Serine-Threonine Kinases / chemistry*
Protein Serine-Threonine Kinases / immunology
Rats
Substrate Specificity

Substances

Epitopes
DNA
Protein Kinases
Glycogen Synthase Kinases
Protein Serine-Threonine Kinases
Calcium-Calmodulin-Dependent Protein Kinases
Glycogen Synthase Kinase 3
tau-protein kinase