RT Journal Article SR Electronic T1 The determination of copper in biological materials by flame spectrophotometry JF Journal of Clinical Pathology JO J Clin Pathol FD BMJ Publishing Group Ltd and Association of Clinical Pathologists SP 181 OP 184 DO 10.1136/jcp.15.2.181 VO 15 IS 2 A1 G. E. Newman A1 M. Ryan YR 1962 UL http://jcp.bmj.com/content/15/2/181.abstract AB A method for the determination of the copper content of biological materials by flame spectrophotometry is described. The effects of interference by ions such as sodium and phosphate were eliminated by isolating copper as the dithizonate in CCl4. Results obtained for the urinary excretion of copper by a patient with Wilson's disease before and after treatment with penicillamine are reported.