RT Journal Article
SR Electronic
T1 Susceptibility testing of Neisseria gonorrhoeae to penicillin and spectinomycin in a diagnostic laboratory.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 978
OP 982
DO 10.1136/jcp.41.9.978
VO 41
IS 9
A1 M J Gill
A1 C A Ison
YR 1988
UL http://jcp.bmj.com/content/41/9/978.abstract
AB Agar dilution breakpoint susceptibility testing using GC, DST, and proteose agars, was performed on consecutive clinical isolates of non-penicillinase producing Neisseria gonorrhoeae to examine the feasibility of using such a system in a diagnostic laboratory. The incidence and level of resistance to penicillin and spectinomycin was also assessed. On DST medium 93 of 200 (46.5%) of isolates were of intermediate resistance to penicillin (MIC 0.12-0.5 mg/l) and 21 of 200 (10.5%) were resistant to penicillin (MIC greater than or equal to 1.0 mg/l). Ninety two of 200 (46%) of isolates had an MIC to spectinomycin of 32 mg/l on DST agar. Isolates seemed to be more resistant when tested on the two other media. The methods used in this study could be applied in a routine diagnostic laboratory for immediate clinical benefit and long term epidemiological studies. To enable direct comparisons to be made between populations at different centres, however, methods for gonococcal susceptibility testing need to be standardised.