RT Journal Article
SR Electronic
T1 Extracting, storing, and transporting whole blood DNA under tropical conditions.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 605
OP 606
DO 10.1136/jcp.44.7.605
VO 44
IS 7
A1 I Bates
A1 G Bedu-Addo
A1 T R Rutherford
YR 1991
UL http://jcp.bmj.com/content/44/7/605.abstract
AB A simple and robust technique for the extraction of DNA under tropical field conditions is described. It requires minimal equipment and is based on lysing cells in whole blood and precipitating the nuclei containing the DNA by centrifugation. The DNA solution can be stored in guanidinium buffer for many months without being refrigerated. Further purification of the DNA can then be carried out in a laboratory with facilities for ultracentrifugation by banding the DNA through cesium chloride. This method yields DNA of sufficient quality and purity for Southern blotting and probing and alleviates the need to transport whole blood between different countries and laboratories.