PT  - JOURNAL ARTICLE
AU  - Paulette Mhawech-Fauceglia
AU  - Francois R Herrmann
AU  - Wiam Bshara
AU  - Kunle Odunsi
AU  - Luigi Terracciano
AU  - Guido Sauter
AU  - Richard T Cheney
AU  - Jeff Groth
AU  - Remedios Penetrante
AU  - Paulette Mhawech-Fauceglia
TI  - Friend leukaemia integration-1 expression in malignant and benign tumours: a multiple tumour tissue microarray analysis using polyclonal antibody
AID  - 10.1136/jcp.2006.039230
DP  - 2007 Jun 01
TA  - Journal of Clinical Pathology
PG  - 694--700
VI  - 60
IP  - 6
4099  - http://jcp.bmj.com/content/60/6/694.short
4100  - http://jcp.bmj.com/content/60/6/694.full
SO  - J Clin Pathol2007 Jun 01; 60
AB  - Background: Friend leukaemia integration-1 (FLI-1) antibody is a useful marker for Ewing’s sarcoma/primitive neuroectodermal tumour (EWS/PNET) and vascular tumours. However, it is also expressed in subsets of lymphoblastic lymphoma, Merkel cell carcinoma (MCC) and desmoplastic small round cell tumour (DSRCT). Aim: To determine expression of FLI-1 in various benign and malignant neoplasms, by immunohistochemical analysis on 4323 tumours using multiple tumour microarrays, as well as on whole sections. Results: FLI-1 was expressed in 46/62 EWS/PNETs, 2/3 olfactory neuroblastomas, 7/102 small cell carcinomas of the lung, 10/34 MCCs, 1/14 rhabdomyosarcoma, 19/132 non-Hodgkin’s lymphomas, 2/3 DSRCTs, and in 53/74 benign and malignant vascular tumours. In addition, 27/508 squamous cell carcinomas, 19/837 adenocarcinomas, 10/400 urothelial bladder cancers, 1/40 basal cell carcinomas, 3/29 liposarcomas, 1/40 glioblastoma multiforme and 9/29 medullar carcinomas of the breast expressed FLI-1. The sensitivity and specificity of FLI-1 to distinguish EWS/PNET from all types of malignancies were 74.2% and 96.0%, respectively. Finally, the sensitivity and specificity of FLI-1 to distinguish EWS/PNET from other small round cell tumours (SRCTs) were 74.2% and 91.6%, respectively. Conclusion: This study was the first to show that FLI-1 can be seen in a variety of solid tumours, some of which had never been explored before. This finding should be kept in mind, especially when using FLI-1 as a marker for finding the primary origin of poorly differentiated metastatic tumour. Finally, despite the expression of FLI-1 in numerous malignancies, it is still considered to be highly sensitive and specific in distinguishing EWS/PNET from other tumour types in general and from other SRCTs in particular.