PT - JOURNAL ARTICLE AU - Björn A Espedido AU - Borce Dimitrijovski AU - Sebastiaan J van Hal AU - Slade O Jensen TI - The use of whole-genome sequencing for molecular epidemiology and antimicrobial surveillance: identifying the role of IncX3 plasmids and the spread of <em>bla</em><sub>NDM-4</sub>-like genes in the Enterobacteriaceae AID - 10.1136/jclinpath-2015-203044 DP - 2015 Oct 01 TA - Journal of Clinical Pathology PG - 835--838 VI - 68 IP - 10 4099 - http://jcp.bmj.com/content/68/10/835.short 4100 - http://jcp.bmj.com/content/68/10/835.full SO - J Clin Pathol2015 Oct 01; 68 AB - Aims To characterise the resistome of a multi-drug resistant Klebsiella pneumoniae (Kp0003) isolated from an Australian traveller who was repatriated to a Sydney Metropolitan Hospital from Myanmar with possible prosthetic aortic valve infective endocarditis.Methods Kp0003 was recovered from a blood culture of the patient and whole genome sequencing was performed. Read mapping and de novo assembly of reads facilitated in silico multi-locus sequence and plasmid replicon typing as well as the characterisation of antibiotic resistance genes and their genetic context. Conjugation experiments were also performed to assess the plasmid (and resistance gene) transferability and the effect on the antibiotic resistance phenotype.Results Importantly, and of particular concern, the carbapenem-hydrolysing β-lactamase gene blaNDM-4 was identified on a conjugative IncX3 plasmid (pJEG027). In this respect, the blaNDM-4 genetic context is similar (at least to some extent) to what has previously been identified for blaNDM-1 and blaNDM-4-like variants.Conclusions This study highlights the potential role that IncX3 plasmids have played in the emergence and dissemination of blaNDM-4-like variants worldwide and emphasises the importance of resistance gene surveillance.