Results of M2-AMA ELISA, ANCA IIF and ANCA Combi-kit® ELISA
| Specimen | M2-AMA ELISA | ANCA IIF pattern (titre) | ANCA Combi-kit® ELISA (OD ratio) | Liver biopsy result |
|---|---|---|---|---|
| ORGenTec ANCA Combi-kit® ELISA OD (optical density) ratio: positive ≥ 1, negative < 1. | ||||
| †Concomitant ANA staining (1/640 titre) with a nuclear membrane pattern was present. | ||||
| #Concomitant ANA staining (1/40 titre) with homogeneous and speckled patterns was present. | ||||
| AICAH, autoimmune chronic active hepatitis; AMA, antimitochondrial antibodies; ANCA, antineutrophil cytoplasmic antibodies; BPI, bactericidal/permeability increasing protein; ELISA, enzyme linked immunosorbent assay; IIF, indirect immunofluorescence; PBC, primary biliary cirrhosis. | ||||
| 1 | High positive | Negative | Anti-BPI (2.47) | PBC |
| 2 | High positive | Negative | Negative | PBC |
| 3 | High positive | Negative | Negative | PBC |
| 4 | High positive | Atypical cytoplasmic (1/40) | Anticathepsin G (1.31) | PBC |
| 5 | High positive | Negative | Antilactoferrin (1.08) | PBC |
| 6 | High positive | Negative | Anti-BPI (1.24) | PBC |
| Antiproteinase 3 (1.13) | ||||
| 7 | High positive | Negative | Anti-BPI (1.79) | PBC |
| 8 | High positive | Negative | Anti-BPI (2.79) | Not done |
| 9 | High positive | Negative | Negative | Not done |
| 10 | High positive | Negative | Negative | Not done |
| 11 | High positive | Perinuclear (1/160) | Anti-BPI (1.60) | Not done |
| Anticathepsin G (2.02) | ||||
| 12 | High positive | Negative | Anti-BPI (1.02) | Not done |
| Antilactoferrin (2.35) | ||||
| 13 | High positive | Perinuclear (1/160) | Anti-BPI (3.04) | Not done |
| Anticathepsin G (2.14) | ||||
| Antilactoferrin (1.6) | ||||
| 14 | High positive | Perinuclear (1/160) | Anti-BPI (1.17) | Not done |
| 15 | High positive | Perinuclear (1/640) | Antilactoferrin (4.37) | Not done |
| 16 | High positive | Perinuclear/nuclear (1/160)† | Anti-BPI (1.30) | Not done |
| 17 | High positive | Negative | Antilactoferrin (1.07) | Not done |
| 18 | High positive | Negative | Antilactoferrin (1.03) | Not done |
| 19 | Mod positive | Perinuclear (1/40) | Antilysozyme (1.41) | PBC |
| 20 | Mod positive | Negative | Negative | AICAH |
| 21 | Mod positive | Perinuclear/nuclear (1/40)# | Anticathepsin G (1.02) | AICAH |
| 22 | Mod positive | Negative | Anticathepsin G (4.24) | Not done |
| 23 | Mod positive | Negative | Negative | Not done |
| 24 | Mod positive | Perinuclear (1/160) | Anti-BPI (4.01) | Not done |
| Anticathepsin G (1.74) | ||||
| Antielastase (1.11) | ||||
| Antilactoferrin (2.12) | ||||
| Antiproteinase-3 (1.05) | ||||
| 25 | Mod positive | Negative | Negative | Not done |
| 26 | Low positive | Atypical cytoplasmic (1/40) | Anticathepsin G (1.00) | PBC |
| 27 | Low positive | Perinuclear (1/160) | Anti-BPI (1.49) | AICAH |
| Anti-elastase (1.46) | ||||
| 28 | Low positive | Negative | Negative | Not done |
| 29 | Low positive | Atypical cytoplasmic (1/40) | Anti-BPI (1.35) | Not done |
| 30 | Low positive | Negative | Negative | Not done |
| 31 | Low positive | Perinuclear | Anti-BPI (1.74) | Not done |
| Anticathepsin G (1.03) | ||||
| 32 | Low positive | Negative | Negative | Not done |